CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005723
UniProt Accession
Genbank Protein ID
 X67241 
Genbank Nucleotide ID
Protein Name
 Ras-specific guanine nucleotide-releasing factor 1 
Protein Synonyms/Alias
 Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; P140 Ras-GRF 
Gene Name
 Rasgrf1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
158ETEKTVAKQLRQQLEubiquitination[1]
204EDEDSDIKKIKKVQSubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Promotes the exchange of Ras-bound GDP by GTP. 
Sequence Annotation
 DOMAIN 22 129 PH 1.
 DOMAIN 204 229 IQ.
 DOMAIN 240 426 DH.
 DOMAIN 456 582 PH 2.
 DOMAIN 629 743 N-terminal Ras-GEF.
 DOMAIN 1009 1241 Ras-GEF.
 MOD_RES 71 71 Phosphoserine; by PLK2.
 MOD_RES 575 575 Phosphoserine; by PLK2.
 MOD_RES 611 611 Phosphoserine; by PLK2.
 MOD_RES 721 721 Phosphoserine (By similarity).
 MOD_RES 760 760 Phosphoserine; by PLK2.
 MOD_RES 781 781 Phosphoserine; by PLK2.
 MOD_RES 854 854 Phosphoserine; by PLK2.  
Keyword
 Complete proteome; Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1244 AA 
Protein Sequence
MQKAIRLNDG HVVSLGLLAQ RDGTRKGYLS KRSSDNPKWQ TKWFALLQNL LFYFESDSSS 60
RPSGLYLLEG SICKRMPSPK RGTSSKESDK QHHYFTVNFS NDSQKSLELR TDDSKDCDEW 120
VAAIARASYK ILATEHEALM QKYLHLLQVV ETEKTVAKQL RQQLEDGEVE IERLKAEIAN 180
LIKDNERIQS NQLVAPEDED SDIKKIKKVQ SFLRGWLCRR KWKNIIQDYI RSPHADSMRK 240
RNQVVFSMLE AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH 300
QIFYQGLKAR IASWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC KQNRDFDKLL 360
KQYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP HEHVERNSLD YAKSKLEELS 420
RVMHDEVSET ENIRKNLAIE RMITEGCEIL LDTSQTFVRQ GSLIQVPMSE KGKINKGRLG 480
SLSLKKEGER QCFLFSKHLI ICTRGSGSKL HLTKNGVISL IDCTLLDDPE NMDDDGKGQE 540
VDHLDFKIWV EPKDSPPFTV ILVASSRQEK AAWTSDIIQC VDNIRCNGLM MNAFEENSKV 600
TVPQMIKSDA SLYCDDVDIR FSKTMNSCKV LQIRYASVER LLERLTDLRF LSIDFLNTFL 660
HSYRVFTDAV VVLDKLISIY KKPITAIPAR SLELLFSSSH NTKLLYGDAP KSPRASRKFS 720
SPPPLAIGTS SPVRRRKLSL NIPIITGGKA LELASLGCPS DGYTNIHSPI SPFGKTTLDT 780
SKLCVASSLT RTPEEIDMTT LEESSGFRKP TSDILKEESD DDQSDVDDTE VSPPTPKSFR 840
NRITQEFPLF NYNSGIMMTC RDLMDSNRSP LSATSAFAIA TAGANESPAN KEIYRRMSLA 900
NTGYSSDQRN IDKEFVIRRA ATNRVLNVLR HWVTKHSQDF ETDDLLKYKV ICFLEEVMHD 960
PDLLPQERKA AANIMRTLTQ EEITENHSML DELLLMTEGV KTEPFENHSA MEIAEQLTLL 1020
DHLVFKSIPY EEFFGQGWMK ADKNERTPYI MKTTRHFNHI SNLIASEILR NEEVSARAST 1080
IEKWVAVADI CRCLHNYNAV LEITSSINRS AIFRLKKTWL KVSKQTKSLF DKLQKLVSSD 1140
GRFKNLRETL RNCDPPCVPY LGMYLTDLAF LEEGTPNYTE DGLVNFSKMR MISHIIREIR 1200
QFQQTTYKIE PQPKVTQYLV DETFVLDDES LYEASLRIEP KLPT 1244 
Gene Ontology
 GO:0005829; C:cytosol; ISS:HGNC.
 GO:0030426; C:growth cone; ISS:HGNC.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005099; F:Ras GTPase activator activity; TAS:UniProtKB.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0032863; P:activation of Rac GTPase activity; ISS:HGNC.
 GO:0031175; P:neuron projection development; ISS:HGNC.
 GO:0046579; P:positive regulation of Ras protein signal transduction; IDA:UniProtKB.
 GO:0035020; P:regulation of Rac protein signal transduction; ISS:HGNC.
 GO:0048167; P:regulation of synaptic plasticity; IDA:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR019804; Ras_G-nucl-exch_fac_CS.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00169; PH
 PF00617; RasGEF
 PF00618; RasGEF_N
 PF00621; RhoGEF 
SMART
 SM00015; IQ
 SM00233; PH
 SM00147; RasGEF
 SM00229; RasGEFN
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50096; IQ
 PS50003; PH_DOMAIN
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER 
PRINTS