UniProt Accession

 NF2IP_HUMAN; Q8NCF5; B7Z4G5; Q66K34; Q6NVK1; Q8NFR2; Q96ST9 

Genbank Protein ID

 AK027545; AK074761; AK297333; AK316535; AF458593; BC018311; BC021551; BC068007; BC080628; BC101741; BC112182 

Genbank Nucleotide ID

 BAB55189.1; BAC11189.1; BAH12551.1; BAH14906.1; AAM49721.1; AAH18311.2; AAH21551.2; AAH68007.1; AAH80628.1; AAI01742.1; AAI12183.1 

Protein Name

 NFATC2-interacting protein 

Protein Synonyms/Alias

 45 kDa NF-AT-interacting protein; 45 kDa NFAT-interacting protein; Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein 

Gene Name

 NFATC2IP 

Gene Synonyms/Alias

 NIP45 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
129	LVPVYSGKVKSSLRL	ubiquitination	[1, 2, 3]
131	PVYSGKVKSSLRLIP	ubiquitination	[2]
268	TPRLFPLKIRCRADL	ubiquitination	[4, 5]
388	AMGLSGRKLSFFFDG	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'- methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity). 

Sequence Annotation

 DOMAIN 348 419 Ubiquitin-like.
 MOD_RES 88 88 Phosphoserine (By similarity).
 MOD_RES 90 90 Phosphoserine (By similarity).
 MOD_RES 92 92 Phosphoserine (By similarity).
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 201 201 Phosphoserine.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 220 220 Phosphoserine.
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 316 316 Phosphothreonine.
 MOD_RES 318 318 Phosphothreonine.
 MOD_RES 369 369 Phosphoserine.
 MOD_RES 390 390 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Methylation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 419 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0001816; P:cytokine production; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:Compara. 

Interpro

 IPR022617; SUMO.
 IPR000626; Ubiquitin.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF11976; Rad60-SLD 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2 

PRINTS