CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024785
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase TRIP12 
Protein Synonyms/Alias
 Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12 
Gene Name
 trip12 
Gene Synonyms/Alias
 si:ch211-272f3.4 
Created Date
 July 27, 2013 
Organism
 Danio rerio (Zebrafish) (Brachydanio rerio) 
NCBI Taxa ID
 7955 
Lysine Modification
Position
Peptide
Type
References
2011SIEIMREKLLIAAREubiquitination[1, 2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardeless of the presence of lysine residues in target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity). 
Sequence Annotation
 DOMAIN 789 876 WWE.
 DOMAIN 1919 2026 HECT.
 REGION 1530 1604 K-box (By similarity).
 ACT_SITE 1993 1993 Glycyl thioester intermediate (By  
Keyword
 Complete proteome; DNA damage; DNA repair; Ligase; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2026 AA 
Protein Sequence
MSNRPNSNPG GSLRRSQRNT AAAQPIDHTL AGRNSLSLSV GSLSIPDPDL EAAGTSGQQG 60
RREGSSTRAL KRSSVSEPNI TFSPSPAKRP KVVSSHFSDS SASGPSAPAI SPEVTEPRKA 120
PASVSTKSKK RRLAAEPAPA RALSKKSSSY PGPSGASSTP SQKRKKADAT LLSSSSSSSL 180
PSSSSAAGPL PPRSEASRAA KPTKLASKSA ASAKAGCSTV TDSSSSSASS SSSSSSSSSA 240
ATGTNSCAPQ GARLKQGKDQ SKARRSRSAS SPSPRRSSRD KEQSKAAGSS KFDWASRFNS 300
KVNLPKPKLS LPGSAKAETS SKPGPSGLQA KLASLRKSTK KRSESPPAEL PSCRRSTRQK 360
TTGSCASTSR RGSGLGKRGA AEARRQEKMA DSDNNQDGAN SSAARTEETP QGASASSSVA 420
GAVGMTTSGE SESDDSEMGR LQALLEARGL PPHLFGPFGP RMSQLFHRTI GSGASSKAQQ 480
LLQGLQATGD ESQQLQAAIE MCQLLVMGNE ETLGGFPVKS VVPALITLLQ MEHNFDIMNH 540
ASRALTYMME ALPRSSAVVV DAIPVFLEKL QVIQFIDVAE QALTALEMLS RRHSKAILQA 600
GGLADCLLYL EFFSINAQRN ALAIAANCCQ SITPDEFHFV ADSLPLLTQR LTHQDKKSVE 660
STCLCFARLV DNFQHEENLL QQVASRDLLT NIQQLLVLTP PVLSSGMFIM VVRMFSLMCS 720
NCPCLAVQLM KQNIAETLRF LLCGASNGSC QEQIDLVPRS PQELYELTSL ICELMPCLPR 780
EGIFAVDAML KKGSAQTTEG AIWQWRDDRG LWHPYNRIDS RIIEQINEDT GTARAIQRKP 840
NPLANPNTGG HLEVRREDAR AQLMKEDPEL AKCFIKTLFG VLYEVYSSSA GPAVRHKCLR 900
AILRIIYFAD AELLKDVLRN HAVSSHIASM LSSQDLKIVV GSLQMAEILM QKLPDVFSVY 960
FRREGVMHQV KNLAESEVFL TSPPKACTSG TASLCTTTIT TATTTAASNV TPDLGSPSFQ 1020
HSMDDSLDLS PQGRLSDVLK RKRLPKRGPR RPKYSPPRDD DKVDNQAKSP TTTQSPKSSF 1080
LASLNPKTWG KLGTQANSAN SEPSRTAGVS GLARVPPKDS VSNNRDKIKA WIKEQASKFV 1140
ERYFNSESVD GSNPALNVLQ RLCTATEQLN LQMDSGVECL EEISSIVSES DVSSFEIQHS 1200
GLVKQLLLYL TSNSERDTIS RDERIKRFLH VFFGCPIPGQ EPPGRLDPTE NGPLLALVHK 1260
MNNCLSQMEQ FPVKVHDFPS GNGNGSRGSQ ALKFFNTHQL KCQLQRHPDC TNVKQWKGGP 1320
VKIDPLALVQ AIERYLVVRG YGRIREEDED SDDDGSDDEI DESLAAQFLN SGSVRHRLQF 1380
YIGEHLLPYN MTVYQAVRQF SLQAEEERES TDDEANPLGR AGIWTKTHTV WYKPVREDEE 1440
GCKDAVGGKR GRAQTAPTKT SPRNAKKQDE LWHEGVCPSV ANPLETYLIC DPPEGITFDD 1500
PSMEVILLLR VLHSISRYWF YLYDNAACKE IIPTGEFINS KLTAKANRQL QDPLVIMTGN 1560
IPTWLTELGK TCPFFFPFDT RQMLFYVTAF DRDRAMQRLL DTNPEINQSD SQDSRVAPRL 1620
DRKKRTINRD ELLKQAESVM QDLGSSRAML EIQYENEVGT GLGPTQEFYA LVSQELQRAD 1680
LGLWRGEEVT LSNPKGSQEG TKYMFSSRGL FAVPFGRTTK PAHIAKIKMK FRFLGKLMAK 1740
AIMDFRLLDL PLGLPFYKWM LRHESSISSH DLVNIDPGVA KSIQHLEDII RQKKRIEQDR 1800
SHTRETLQQA LESLNMNGCS VEDLGLDFTL PGFPNIELKK GGKDVPVTIH NLEDYLRLVV 1860
YWTLNEGVLR QFESFREGFE SVFPLHHLQY FYPEELDQLL CGSKSESWDV KTLMECCRPD 1920
HGYTHDSRAV RFLFEVLSSF DAEQQRLFLQ FVTGSPRLPV GGFRSLNPPL TIVRKTFEST 1980
ENPDDFLPSV MTCVNYLKLP DYSSIEIMRE KLLIAAREGQ QSFHLS 2026 
Gene Ontology
 GO:0005654; C:nucleoplasm; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0009790; P:embryo development; ISS:UniProtKB.
 GO:2000780; P:negative regulation of double-strand break repair; ISS:UniProtKB.
 GO:1901315; P:negative regulation of histone H2A K63-linked ubiquitination; ISS:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; ISS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000569; HECT.
 IPR004170; WWE-dom. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT
 PS50918; WWE 
PRINTS