UniProt Accession

 PTH2_YEAST; P34222; D6VPU3 

Genbank Protein ID

 Z23261; Z35818; AY692763; BK006936 

Genbank Nucleotide ID

 CAA80790.1; CAA84877.1; AAT92782.1; DAA07063.2 

Protein Name

 Peptidyl-tRNA hydrolase 2 

Protein Synonyms/Alias

 PTH 2 

Gene Name

 PTH2 

Gene Synonyms/Alias

 YBL057C; YBL0510; YBL0514 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
152	ITLKCPDKFTMDELY	ubiquitination	[1, 2, 3]
161	TMDELYAKAISLGVN	ubiquitination	[1]
206	DQITGDLKLY*****	ubiquitination	[3]

Reference

 [1] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis (By similarity). 

Sequence Annotation

 CROSSLNK 152 152 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Isopeptide bond; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 208 AA 

Protein Sequence

Gene Ontology

 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0004045; F:aminoacyl-tRNA hydrolase activity; IDA:SGD.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD. 

Interpro

 IPR023476; Pep_tRNA_hydro_II_dom.
 IPR002833; Pep_tRNA_hydro_PTH2. 

Pfam

 PF01981; PTH2 

SMART

  

PROSITE

  

PRINTS