CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005520
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytoplasmic alpha-amylase 
Protein Synonyms/Alias
 1,4-alpha-D-glucan glucanohydrolase 
Gene Name
 amyA 
Gene Synonyms/Alias
 yedC; b1927; JW1912 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
112HKMGADEKEAIRVQRacetylation[1]
217HAVTEEIKYWARWVMacetylation[1]
238GFRLDAVKHIPAWFYacetylation[1]
456LGENYGNKTWRDFLGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 ACT_SITE 235 235 Nucleophile (By similarity).
 ACT_SITE 265 265 Proton donor (By similarity).
 METAL 104 104 Calcium (By similarity).
 METAL 239 239 Calcium; via carbonyl oxygen (By  
Keyword
 Calcium; Carbohydrate metabolism; Complete proteome; Cytoplasm; Glycosidase; Hydrolase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 495 AA 
Protein Sequence
MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG GYSVGYDSYD 60
LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD VVVNHKMGAD EKEAIRVQRV 120
NADDRTQIDE EIIECEGWTR YTFPARAGQY SQFIWDFKCF SGIDHIENPD EDGIFKIVND 180
YTGEGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI 240
PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS 300
RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN 360
GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL ARQRFAHGVQ TLFFDHPNCI 420
AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG ENYGNKTWRD FLGNRQERVV TDENGEATFF 480
CNGGSVSVWV IEEVI 495 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0004556; F:alpha-amylase activity; IDA:EcoCyc.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR013776; A-amylase_thermo.
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR006589; Glyco_hydro_13_sub_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00128; Alpha-amylase 
SMART
 SM00642; Aamy 
PROSITE
  
PRINTS