| Tag | Content |
|---|
| CPLM ID | CPLM-004798 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 15 |
Protein Synonyms/Alias | Autonomous replication regulatory protein 3; Basal expression activator protein 1; Defective silencing suppressor protein 4; Mediator complex subunit 15; Transcription regulatory protein GAL11; Ty insertion suppressor protein 13 |
Gene Name | GAL11 |
Gene Synonyms/Alias | ABE1; MED15; RAR3; SDS4; SPT13; YOL051W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 590 | FVKEILEKCAEGIFV | ubiquitination | [1] | | 599 | AEGIFVVKLDTVDRL | ubiquitination | [1] |
|
Reference | [1] Identification, analysis, and prediction of protein ubiquitination sites. Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM. Proteins. 2010 Feb 1;78(2):365-80. [ PMID: 19722269] |
Functional Description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription. |
Sequence Annotation | REPEAT 422 423 1.
REPEAT 424 425 2.
REPEAT 426 427 3.
REPEAT 428 429 4.
REPEAT 430 431 5.
REPEAT 432 433 6.
REPEAT 434 435 7.
REPEAT 436 437 8.
REPEAT 438 439 9.
REPEAT 440 441 10.
REPEAT 442 443 11.
REPEAT 444 445 12; approximate.
REPEAT 446 447 13; approximate.
REPEAT 448 449 14.
REPEAT 450 451 15.
REPEAT 452 453 16.
REPEAT 454 455 17.
REPEAT 456 457 18.
REPEAT 458 459 19.
REPEAT 460 461 20.
REPEAT 462 463 21.
REPEAT 464 465 22.
REPEAT 466 467 23.
REPEAT 468 469 24.
REPEAT 470 471 25.
REPEAT 472 473 26.
REPEAT 474 475 27.
REPEAT 476 477 28.
REPEAT 478 479 29.
REPEAT 480 481 30.
REGION 422 481 30 X 2 AA approximate tandem repeats of
MOD_RES 335 335 Phosphoserine.
MOD_RES 736 736 Phosphoserine.
MOD_RES 752 752 Phosphoserine.
MOD_RES 783 783 Phosphoserine.
MOD_RES 785 785 Phosphoserine.
MOD_RES 789 789 Phosphoserine.
MOD_RES 793 793 Phosphothreonine.
MOD_RES 831 831 Phosphoserine.
MOD_RES 1003 1003 Phosphoserine.
MOD_RES 1008 1008 Phosphoserine.
MOD_RES 1018 1018 Phosphoserine.
MOD_RES 1034 1034 Phosphoserine. |
Keyword | 3D-structure; Activator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1081 AA |
Protein Sequence | MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK NFEAALFAKS 60
SSKKEYMDSM NEKVAVMRNT YNTRKNAVTA AAANNNIKPV EQHHINNLKN SGNSANNMNV 120
NMNLNPQMFL NQQAQARQQV AQQLRNQQQQ QQQQQQQQRR QLTPQQQQLV NQMKVAPIPK 180
QLLQRIPNIP PNINTWQQVT ALAQQKLLTP QDMEAAKEVY KIHQQLLFKA RLQQQQAQAQ 240
AQANNNNNGL PQNGNINNNI NIPQQQQMQP PNSSANNNPL QQQSSQNTVP NVLNQINQIF 300
SPEEQRSLLQ EAIETCKNFE KTQLGSTMTE PVKQSFIRKY INQKALRKIQ ALRDVKNNNN 360
ANNNGSNLQR AQNVPMNIIQ QQQQQNTNNN DTIATSATPN AAAFSQQQNA SSKLYQMQQQ 420
QQAQAQAQAQ AQAQAQAQAQ AQAAQAAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ 480
AHAQHQPSQQ PQQAQQQPNP LHGLTPTAKD VEVIKQLSLD ASKTNLRLTD VTNSLSNEEK 540
EKIKMKLKQG QKLFVQVSNF APQVYIITKN ENFLKEVFQL RIFVKEILEK CAEGIFVVKL 600
DTVDRLIIKY QKYWESMRIQ ILRRQAILRQ QQQMANNNGN PGTTSTGNNN NIATQQNMQQ 660
SLQQMQHLQQ LKMQQQQQQQ QQQQQQQQQQ QQQQQQHIYP SSTPGVANYS AMANAPGNNI 720
PYMNHKNTSS MDFLNSMENT PKVPVSAAAT PSLNKTINGK VNGRTKSNTI PVTSIPSTNK 780
KLSISNAASQ QPTPRSASNT AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN 840
SAIIENAFRK EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLGD CLGIKDEEML 900
TSCTIPKAVV DHINGSGKRK PTKAAQRARD QDSIDISIKD NKLVMKSKFN KSNRSYSIAL 960
SNVAAIFKGI GGNFKDLSTL VHSSSPSTSS NMDVGNPRKR KASVLEISPQ DSIASVLSPD 1020
SNIMSDSKKI KVDSPDDPFM TKSGATTSEK QEVTNEAPFL TSGTSSEQFN VWDWNNWTSA 1080
T 1081 |
Gene Ontology | GO:0070847; C:core mediator complex; IDA:SGD. GO:0016592; C:mediator complex; IEA:InterPro. GO:0001102; F:RNA polymerase II activating transcription factor binding; IDA:SGD. GO:0001128; F:RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly; IDA:SGD. GO:0001135; F:RNA polymerase II transcription factor recruiting transcription factor activity; IMP:SGD. GO:0001088; F:TFIIE-class binding transcription factor activity; IDA:SGD. GO:0001095; F:TFIIE-class transcription factor binding; IDA:SGD. GO:0001097; F:TFIIH-class transcription factor binding; IDA:SGD. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |