UniProt Accession

 B4DM58_HUMAN; B4DM58 

Genbank Protein ID

 AC008434; AC010270; AK297302 

Genbank Nucleotide ID

 BAG59770.1 

Protein Name

 Dihydrofolate reductase 

Protein Synonyms/Alias

 cDNA FLJ51500, highly similar to Dihydrofolate reductase (EC 1.5.1.3) 

Gene Name

 DHFR 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	SQNMGIGKNGDLPWP	ubiquitination	[1, 2]
47	TTSSVEGKQNLVIMG	ubiquitination	[1, 3]
55	QNLVIMGKKTWFSIP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 129 AA 

Protein Sequence

Gene Ontology

 GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro. 

Interpro

 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR017925; DHFR_CS.
 IPR001796; DHFR_dom. 

Pfam

 PF00186; DHFR_1 

SMART

  

PROSITE

 PS00075; DHFR_1
 PS51330; DHFR_2 

PRINTS

 PR00070; DHFR.