CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008536
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribonuclease UK114 
Protein Synonyms/Alias
 Heat-responsive protein 12 
Gene Name
 Hrsp12 
Gene Synonyms/Alias
 Hrp12 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
7*MSSIIRKVISTTKAacetylation[1]
7*MSSIIRKVISTTKAsuccinylation[1]
13RKVISTTKAPAAIGPacetylation[1]
13RKVISTTKAPAAIGPsuccinylation[1]
13RKVISTTKAPAAIGPubiquitination[2]
60EEAKQALKNLGEILKacetylation[1]
60EEAKQALKNLGEILKsuccinylation[1]
60EEAKQALKNLGEILKubiquitination[2]
67KNLGEILKAAGCDFNacetylation[1, 3, 4, 5]
67KNLGEILKAAGCDFNsuccinylation[1]
67KNLGEILKAAGCDFNubiquitination[2]
97GTVNEIYKTYFQGSLacetylation[1]
97GTVNEIYKTYFQGSLsuccinylation[1]
134AVQGPFIKA******acetylation[1, 6]
134AVQGPFIKA******succinylation[1]
134AVQGPFIKA******ubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379
Functional Description
 Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 21 21 Phosphotyrosine.  
Keyword
 Acetylation; Complete proteome; Endonuclease; Hydrolase; Nuclease; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 135 AA 
Protein Sequence
MSSIIRKVIS TTKAPAAIGP YSQAVQVDRT IYISGQVGLD PSSGQLVPGG VVEEAKQALK 60
NLGEILKAAG CDFNNVVKTT VLLADMNDFG TVNEIYKTYF QGSLPARAAY QVAALPRGSR 120
VEIEAIAVQG PFIKA 135 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0019239; F:deaminase activity; IEA:InterPro.
 GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 
Interpro
 IPR013813; Endoribo_LPSP/chorism_mut-like.
 IPR006056; YjgF-like.
 IPR019897; YjgF-like_CS.
 IPR006175; YjgF/Yer057p/UK114. 
Pfam
 PF01042; Ribonuc_L-PSP 
SMART
  
PROSITE
 PS01094; UPF0076 
PRINTS