UniProt Accession

 CRYAA_HUMAN; P02489; Q53X53 

Genbank Protein ID

 U05569; U66584; X14789; CR407691; AP001631; AP001748; CH471079; CH471079; BC069528; BC113598; M35628; M35629 

Genbank Nucleotide ID

 AAA97523.1; AAC50900.1; CAA32891.1; CAG28619.1; BAA95535.1; EAX09497.1; EAX09498.1; AAH69528.1; AAI13599.1; AAA52106.1; AAA52105.1 

Protein Name

 Alpha-crystallin A chain 

Protein Synonyms/Alias

 Heat shock protein beta-4; HspB4; Alpha-crystallin A chain, short form 

Gene Name

 CRYAA 

Gene Synonyms/Alias

 CRYA1; HSPB4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	TIQHPWFKRTLGPFY	methylation	[1]
70	EVRSDRDKFVIFLDV	acetylation	[2, 3, 4]
78	FVIFLDVKHFSPEDL	acetylation	[3]
88	SPEDLTVKVQDDFVE	acetylation	[3]
88	SPEDLTVKVQDDFVE	methylation	[3, 5]
99	DFVEIHGKHNERQDD	acetylation	[4]
145	MLTFCGPKIQTGLDA	acetylation	[3]

Reference

 [1] Age-related changes in human crystallins determined from comparative analysis of post-translational modifications in young and aged lens: does deamidation contribute to crystallin insolubility?
 Wilmarth PA, Tanner S, Dasari S, Nagalla SR, Riviere MA, Bafna V, Pevzner PA, David LL.
 J Proteome Res. 2006 Oct;5(10):2554-66. [PMID: 17022627]
 [2] In vivo acetylation identified at lysine 70 of human lens alphaA-crystallin.
 Lin PP, Barry RC, Smith DL, Smith JB.
 Protein Sci. 1998 Jun;7(6):1451-7. [PMID: 9655350]
 [3] Shotgun identification of protein modifications from protein complexes and lens tissue.
 MacCoss MJ, McDonald WH, Saraf A, Sadygov R, Clark JM, Tasto JJ, Gould KL, Wolters D, Washburn M, Weiss A, Clark JI, Yates JR 3rd.
 Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7900-5. [PMID: 12060738]
 [4] Acetylation of αA-crystallin in the human lens: effects on structure and chaperone function.
 Nagaraj RH, Nahomi RB, Shanthakumar S, Linetsky M, Padmanabha S, Pasupuleti N, Wang B, Santhoshkumar P, Panda AK, Biswas A.
 Biochim Biophys Acta. 2012 Feb;1822(2):120-9. [PMID: 22120592]
 [5] Update on activities at the Universal Protein Resource (UniProt) in 2013.
 e="String">UniProt Consortium.
 Nucleic Acids Res. 2013 Jan;41(Database issue):D43-7. [PMID: 23161681] 

Functional Description

 May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. 

Sequence Annotation

 METAL 79 79 Zinc.
 METAL 107 107 Zinc.
 METAL 115 115 Zinc.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 70 70 N6-acetyllysine.
 MOD_RES 99 99 N6-acetyllysine.
 MOD_RES 101 101 Deamidated asparagine; partial.
 MOD_RES 122 122 Phosphoserine.
 CARBOHYD 162 162 O-linked (GlcNAc) (By similarity).
 DISULFID 131 142  

Keyword

 Acetylation; Cataract; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Disulfide bond; Eye lens protein; Glycoprotein; Metal-binding; Nucleus; Oxidation; Phosphoprotein; Polymorphism; Reference proteome; Sensory transduction; Vision; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 173 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005212; F:structural constituent of eye lens; IEA:UniProtKB-KW.
 GO:0051082; F:unfolded protein binding; IMP:UniProtKB.
 GO:0007015; P:actin filament organization; IEA:Compara.
 GO:0060561; P:apoptotic process involved in morphogenesis; IEA:Compara.
 GO:0048596; P:embryonic camera-type eye morphogenesis; IEA:Compara.
 GO:0070309; P:lens fiber cell morphogenesis; IEA:Compara.
 GO:0000072; P:M phase specific microtubule process; IEA:Compara.
 GO:0007005; P:mitochondrion organization; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0032387; P:negative regulation of intracellular transport; IDA:HGNC.
 GO:0030307; P:positive regulation of cell growth; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:0006457; P:protein folding; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0042542; P:response to hydrogen peroxide; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0010288; P:response to lead ion; IEA:Compara.
 GO:0070141; P:response to UV-A; IEA:Compara.
 GO:0007021; P:tubulin complex assembly; IEA:Compara.
 GO:0007601; P:visual perception; IMP:UniProtKB. 

Interpro

 IPR002068; a-crystallin/Hsp20_dom.
 IPR001436; Alpha-crystallin/HSP.
 IPR012274; Alpha-crystallin_A.
 IPR003090; Alpha-crystallin_N.
 IPR008978; HSP20-like_chaperone. 

Pfam

 PF00525; Crystallin
 PF00011; HSP20 

SMART

  

PROSITE

 PS01031; HSP20 

PRINTS

 PR00299; ACRYSTALLIN.