UniProt Accession

 BE_THET8; Q5SH28; P84162 

Genbank Protein ID

 AP008226 

Genbank Nucleotide ID

 BAD71725.1 

Protein Name

 1,4-alpha-glucan branching enzyme TTHA1902 

Protein Synonyms/Alias

 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Alpha-(1->4)-glucan branching enzyme; Branching enzyme; BE 

Gene Name

 TTHA1902; TT1467 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) 

NCBI Taxa ID

 300852 

Lysine Modification

Position	Peptide	Type	References
490	RAFFHLLKGASPEEL	acetylation	[1]

Reference

 [1] Acetylome with structural mapping reveals the significance of lysine acetylation in Thermus thermophilus.
 Okanishi H, Kim K, Masui R, Kuramitsu S.
 J Proteome Res. 2013 Aug 1;. [PMID: 23901841] 

Functional Description

 Catalyzes the formation of branch points in alpha- glucans by cleavage of an alpha-1,4 glycosidic bond and subsequent transfer of the cleaved-off oligosaccharide to a new alpha-1,6 position. The branch chain-length distribution of the reaction products shows degree of polymerization (DP) of 3 to 13, with two local maxima at DP 7 and DP 11. Exhibits an alpha-retaining catalytic mechanism. Is involved in glycogen biosynthesis. Shows a secondary activity, i.e. the hydrolysis of the substrate, being 4% of the total activity. Can use amylose as substrate but not alpha- 1,4-linked oligosaccharides of 2-7 glucose residues, beta- cyclodextrin, 6-O-glucosyl-beta-cyclodextrin and 6-O-maltosyl- beta-cyclodextrin. Is not able to branch amylopectin further, it only hydrolyzes amylopectin. Thus, displays preference for linear and long substrates (amylose) over branched structures (amylopectin). 

Sequence Annotation

 ACT_SITE 184 184 Nucleophile.
 ACT_SITE 353 353 Proton donor.
 BINDING 265 265 Substrate (By similarity).
 BINDING 282 282 Substrate; via amide nitrogen (By
 BINDING 404 404 Substrate (By similarity).
 BINDING 460 460 Substrate (By similarity).
 BINDING 469 469 Substrate (By similarity).  

Keyword

 3D-structure; Carbohydrate metabolism; Complete proteome; Glycogen biosynthesis; Glycogen metabolism; Glycosyltransferase; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 520 AA 

Protein Sequence

Gene Ontology

 GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IDA:UniProtKB.
 GO:0005978; P:glycogen biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR015293; DUF1957.
 IPR011330; Glyco_hydro/deAcase_b/a-brl.
 IPR027291; Glyco_hydro_38/57_N.
 IPR004300; Glyco_hydro_57_N. 

Pfam

 PF09210; DUF1957
 PF03065; Glyco_hydro_57 

SMART

  

PROSITE

  

PRINTS