CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023603
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication complex GINS protein PSF2 
Protein Synonyms/Alias
 GINS complex subunit 2 
Gene Name
 GINS2 
Gene Synonyms/Alias
 PSF2; CGI-122; DC5; HSPC037 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70PEWMDVEKLEKMRDHubiquitination[1]
73MDVEKLEKMRDHERKubiquitination[1]
80KMRDHERKEETFTPMubiquitination[1, 2, 3]
109HASDNIPKADEIRTLubiquitination[1, 2]
118DEIRTLVKDMWDTRIubiquitination[1, 2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single- stranded DNA. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 180 180 Phosphothreonine.
 MOD_RES 182 182 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Complete proteome; DNA replication; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 185 AA 
Protein Sequence
MDAAEVEFLA EKELVTIIPN FSLDKIYLIG GDLGPFNPGL PVEVPLWLAI NLKQRQKCRL 60
LPPEWMDVEK LEKMRDHERK EETFTPMPSP YYMELTKLLL NHASDNIPKA DEIRTLVKDM 120
WDTRIAKLRV SADSFVRQQE AHAKLDNLTL MEINTSGTFL TQALNHMYKL RTNLQPLEST 180
QSQDF 185 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 
Interpro
 IPR021151; GINS_complex.
 IPR007257; GINS_Psf2.
 IPR016906; GINS_Psf2_subgr. 
Pfam
 PF05916; Sld5 
SMART
  
PROSITE
  
PRINTS