CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015956
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neuroepithelial cell-transforming gene 1 protein 
Protein Synonyms/Alias
 Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8 
Gene Name
 NET1 
Gene Synonyms/Alias
 ARHGEF8 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9EPELAAQKQPRPRRRubiquitination[1]
137RSPASAQKFSSRSTVubiquitination[1]
149STVPTPAKRRSSALWubiquitination[1, 2]
194QDLIEDLKLARKAYHubiquitination[1, 3, 4, 5]
198EDLKLARKAYHDPMLubiquitination[1]
241TRIGEATKPDGTVEQubiquitination[1]
274CSNQLAAKALLDQKKubiquitination[1, 5]
280AKALLDQKKQDPRVQubiquitination[1]
301LESPFSRKLDLWSFLubiquitination[1]
317IPRSRLVKYPLLLKEubiquitination[1]
357LSDINLKKGESECQYubiquitination[1]
368ECQYYIDKLEYLDEKubiquitination[1]
385DPRIEASKVLLCHGEubiquitination[1]
489QANDVFHKQQWFNCIubiquitination[1]
567MQMAEDSKSLKTHQTubiquitination[1]
570AEDSKSLKTHQTQPGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 
Sequence Annotation
 DOMAIN 174 356 DH.
 DOMAIN 386 501 PH.
 REGION 1 74 Necessary for nuclear localization (By
 MOTIF 12 19 Nuclear localization signal (By
 MOTIF 66 72 Nuclear localization signal (By
 MOD_RES 21 21 Phosphoserine.
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 508 508 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Nucleus; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 596 AA 
Protein Sequence
MEPELAAQKQ PRPRRRSRRA SGLSTEGATG PSADTSGSEL DGRCSLRRGS SFTFLTPGPN 60
WDFTLKRKRR EKDDDVVSLS SLDLKEPSNK RVRPLARVTS LANLISPVRN GAVRRFGQTI 120
QSFTLRGDHR SPASAQKFSS RSTVPTPAKR RSSALWSEML DITMKESLTT REIRRQEAIY 180
EMSRGEQDLI EDLKLARKAY HDPMLKLSIM SEEELTHIFG DLDSYIPLHE DLLTRIGEAT 240
KPDGTVEQIG HILVSWLPRL NAYRGYCSNQ LAAKALLDQK KQDPRVQDFL QRCLESPFSR 300
KLDLWSFLDI PRSRLVKYPL LLKEILKHTP KEHPDVQLLE DAILIIQGVL SDINLKKGES 360
ECQYYIDKLE YLDEKQRDPR IEASKVLLCH GELRSKSGHK LYIFLFQDIL VLTRPVTRNE 420
RHSYQVYRQP IPVQELVLED LQDGDVRMGG SFRGAFSNSE KAKNIFRIRF HDPSPAQSHT 480
LQANDVFHKQ QWFNCIRAAI APFQSAGSPP ELQGLPELHE ECEGNHPSAR KLTAQRRAST 540
VSSVTQVEVD ENAYRCGSGM QMAEDSKSLK THQTQPGIRR ARDKALSGGK RKETLV 596 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0070301; P:cellular response to hydrogen peroxide; IDA:UniProtKB.
 GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0032321; P:positive regulation of Rho GTPase activity; IMP:UniProtKB.
 GO:0001558; P:regulation of cell growth; NAS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015721; RhoGEF-like. 
Pfam
 PF00169; PH
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS