UniProt Accession

 ASNS_MOUSE; Q61024; Q3TJA1; Q8BPC8 

Genbank Protein ID

 U38940; AK076207; AK167524; BC005552 

Genbank Nucleotide ID

 AAA85125.1; BAC36254.1; BAE39594.1; AAH05552.1 

Protein Name

 Asparagine synthetase [glutamine-hydrolyzing] 

Protein Synonyms/Alias

 Glutamine-dependent asparagine synthetase 

Gene Name

 Asns 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
350	VGMYLISKYIRKNTD	acetylation	[1]
395	EESERLLKELYLFDV	acetylation	[1]
395	EESERLLKELYLFDV	ubiquitination	[2]
478	SDGITSVKNSWFKIL	ubiquitination	[2]
513	PFNTPKTKEGYFYRQ	acetylation	[1]
556	ARTLTHYKSAAKA**	acetylation	[3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

  

Sequence Annotation

 DOMAIN 2 191 Glutamine amidotransferase type-2.
 DOMAIN 213 536 Asparagine synthetase.
 NP_BIND 363 364 ATP (By similarity).
 REGION 49 53 Glutamine binding (By similarity).
 REGION 75 77 Glutamine binding (By similarity).
 ACT_SITE 2 2 For GATase activity (By similarity).
 BINDING 97 97 Glutamine (By similarity).
 BINDING 256 256 ATP; via carbonyl oxygen (By similarity).
 BINDING 288 288 ATP; via amide nitrogen and carbonyl
 MOD_RES 385 385 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 561 AA 

Protein Sequence

Gene Ontology

 GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0048037; F:cofactor binding; IEA:Compara.
 GO:0042149; P:cellular response to glucose starvation; IEA:Compara.
 GO:0032870; P:cellular response to hormone stimulus; IEA:Compara.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0070981; P:L-asparagine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0045931; P:positive regulation of mitotic cell cycle; IEA:Compara.
 GO:0043200; P:response to amino acid stimulus; IEA:Compara.
 GO:0032354; P:response to follicle-stimulating hormone stimulus; IEA:Compara.
 GO:0009416; P:response to light stimulus; IEA:Compara.
 GO:0009612; P:response to mechanical stimulus; IEA:Compara.
 GO:0031427; P:response to methotrexate; IEA:Compara.
 GO:0009636; P:response to toxic substance; IEA:Compara. 

Interpro

 IPR006426; Asn_synth_AEB.
 IPR001962; Asn_synthase.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF00733; Asn_synthase
 PF13537; GATase_7 

SMART

  

PROSITE

 PS51278; GATASE_TYPE_2 

PRINTS