CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007693
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome activator BLM10 
Protein Synonyms/Alias
 Bleomycin resistance protein BLM10 
Gene Name
 BLM10 
Gene Synonyms/Alias
 BLM3; YFL007W; YFL006W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
18SPIPITNKTLSQLKRacetylation[1]
180EVDDLALKTDLFRLSacetylation[1]
493HSYSRTVKPFVYAINacetylation[1]
532SNNGFWTKPNAKFVHacetylation[1]
1076IEQIRSGKDDEDYIEubiquitination[2]
2042AKGLDVNKYTSKERQubiquitination[2]
2119KVRADTWKFDRASFNacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin- independent degradation of core histones during DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Involved in DNA damage response in somatic cells: binds to acetylated histones and promotes degradation of histones following DNA double-strand breaks. 
Sequence Annotation
 REPEAT 1316 1355 HEAT 1.
 REPEAT 1779 1814 HEAT 2.
 REPEAT 1902 1941 HEAT 3.
 REPEAT 1985 2023 HEAT 4.
 REGION 1648 1732 Bromodomain-like (BRDL).
 MOTIF 2141 2143 YYX motif.
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 29 29 Phosphoserine.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 62 62 Phosphoserine.
 MOD_RES 64 64 Phosphothreonine.
 MOD_RES 66 66 Phosphothreonine.
 MOD_RES 1041 1041 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2143 AA 
Protein Sequence
MTANNDDDIK SPIPITNKTL SQLKRFERSP GRPSSSQGEI KRKKSRLYAA DGRPHSPLRA 60
RSATPTLQDQ KLFNGMDSTS LLNERLQHYT LDYVSDRAQH MKNIYDPSSR WFSRSVRPEF 120
PIEEFLPYKT ESHEDQAKYL CHVLVNLYIA ISSLDIQGLI SISSKDLADL KKEVDDLALK 180
TDLFRLSNNT AENDLLGNDI ADYDDAEGLE DELDEYFDLA GPDFNATGKI TAKSATIVNV 240
NHWTNELKNC LHFDFPVALR KSLATVYYYL SLVQGQKVYR QMHVDMFERL VSLDDDRTNF 300
TELLQKQGLL LDHQIMLNFL CEFLPYPDPD YARYELSSKE DLQLFRLLLK HAHNAKPFFD 360
KSKESLLVDT MNFLLSSLAP STMMAVMPIV TSVVPYHYHI HSKIIDYFPF CYSIWSSVSA 420
NVAIDTHMYD FVGSISKDVH NKILSSEHEK DVVGVEFGEF GIFTDDQMTF MFNRLQGHLR 480
TDGQIHSYSR TVKPFVYAIN GSKKDRFFEK LVSLAKAIET FIHPSNNGFW TKPNAKFVHA 540
FIKSYHGRVK YEEDICARGV TNGICLTSFC HEEIVEIFLN IISLGSQNKN PDIANYYISC 600
FAYLLELDPS NAYLIYDKIL IDLYDTLADQ FINSRHRIIS SLKQFTRVIR FIVMDKLYRV 660
HITNVLSMLV SKLDMNDTNL TSNLINGIVS IAAFIPIQDL TGEDDYISFE SDTLPLVQQH 720
FYHIKCGESS KTFRVDDELL NNAFKASTTV FQSMLKVYVE KIFQLVDVDL EDSLVTKINQ 780
TTMILQESMD DKIFNYFASL LQRNFWSNDS FKEKDPNYEL VTIPLAALVR RNNGLSKELV 840
RTLLFHIKEQ IKRGAGSVRS TSEIQQRDVK LVLYLTALND VLRQCHESLL EYSDELITFM 900
KYLYDNVTNP PLDVITSIVI HSALATLCTT EITDCRLFPE DSKIPEKDRW GGLQFDPRRF 960
DKQHLSFQWH VPSSDEITLS ISILESLSEY CINNVEELMK APRHDSEYGD MIQKYVLVMT 1020
HTLSGSSLLF DPDFNKYRTQ SNLSYREKLI LLKNIRENNC DPQELDIDIE QIRSGKDDED 1080
YIESKDIEAG LNAGVSDVVQ LRDEFPDELI VDEEVVSEMP SGVNTPIAGT HGTDNSAMSS 1140
DLAFRDLDIY TCNYYFGNTT EEKLQNPQYL QVHRVRARIG HFFHKLYVFL STNFENNTNM 1200
FQILLHGLKV WFTDLGQETV FNEDPNAFID VDFLENVQSL SHVNEPFTRT NFAIRANSLH 1260
QSRVLLHSTN RKASKLENLL LVDIIQLATS LYPDIYKPAQ GTLVHCMKQL VGSYGVVINK 1320
IIPSLEKAIK DHDYMKIQVI LNVLLIKKIH RKLMTDYKDI GRLIFLLIEC CRVNELEIGM 1380
YADKILTDIV IGIKIPSSVC VISDQAFLPL APPDGTINLQ VEAVKLAKKK KREYYLSLLV 1440
DLQDKLLDKL DNEKDMGWKI RMFILRFVTQ IQSNLESKPD KRAVFSIISQ ISTKHPEIIH 1500
LVVKSLLSTC NKIISLSDYE YDITRAYKNE FNPSFVEILD TSTTSFPKTF TEEMNNFDNP 1560
KYFIDLRAYV GWLCWGRLMY VMSPKALKLN LRENELEVLK TAGHLLTREF LRDVTMNLVQ 1620
DNETRGVFSS GNVSFFSLVI LLISSGFCEL NMSDLFELCE SYYNKDDKAS MIMSVEIVAG 1680
LVCGSKFMSV SDLDKRDTFI ENFLAKCLDY ELNHDAFEIW STLAWWLPAV VDLRRSKTFF 1740
CHFINADGMF DRESDAATHQ TSKIYMLRSI LMSMEFRAPD VGKLFDELVF DHPYDQVRQA 1800
VAKLLTTLVQ NQSNPSISDP TTLLEAERND PDGLGLPLKS VPEKVDAYIK KQFEIIKNLE 1860
DSVVGLNPQQ FIKTDYFYRT STIFYWIKEM ARGPNKVLLV PYLVDYVLPF LIGLVKHKDV 1920
CALASLDPVR LYAGLGYMPI RKNHVAAIVD YVCSSNVALS SNQTKLQLAF IQHFLSAELL 1980
QLTEEEKNKI LEFVVSNLYN EQFVEVRVRA ASILSDIVHN WKEEQPLLSL IERFAKGLDV 2040
NKYTSKERQK LSKTDIKIHG NVLGLGAIIS AFPYVFPLPP WIPKQLSNLS SWARTSGMTG 2100
QAAKNTISEF KKVRADTWKF DRASFNTEEL EDLEGVLWRS YYA 2143 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0016504; F:peptidase activator activity; IDA:SGD.
 GO:0070628; F:proteasome binding; IMP:SGD.
 GO:0043248; P:proteasome assembly; IMP:SGD.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IDA:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021843; DUF3437. 
Pfam
 PF11919; DUF3437 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS