UniProt Accession

 T2FB_RAT; Q01750 

Genbank Protein ID

 L01267 

Genbank Nucleotide ID

 AAA42005.1 

Protein Name

 General transcription factor IIF subunit 2 

Protein Synonyms/Alias

 ATP-dependent helicase GTF2F2; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30 

Gene Name

 Gtf2f2 

Gene Synonyms/Alias

 Rap30 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
42	PGRGEVGKLRIAKNQ	acetylation	[1]
71	NIHDIGGKPASVSAP	acetylation	[1]
137	LQIEESSKPVRLSQQ	acetylation	[1]
154	KVVTTNYKPVANHQY	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity (By similarity). 

Sequence Annotation

 NP_BIND 36 43 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 22 22 N6-acetyllysine (By similarity).
 MOD_RES 33 33 N6-acetyllysine (By similarity).
 MOD_RES 137 137 N6-acetyllysine (By similarity).
 MOD_RES 142 142 Phosphoserine (By similarity).
 MOD_RES 248 248 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 249 AA 

Protein Sequence

Gene Ontology

 GO:0005674; C:transcription factor TFIIF complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:RGD.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro. 

Interpro

 IPR016640; TFIIF-beta_subgr.
 IPR003196; TFIIF_beta.
 IPR011039; TFIIF_interaction.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02270; TFIIF_beta 

SMART

  

PROSITE

  

PRINTS