CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008919
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Delta-1-pyrroline-5-carboxylate synthase 
Protein Synonyms/Alias
 P5CS; Aldehyde dehydrogenase family 18 member A1; Glutamate 5-kinase; GK; Gamma-glutamyl kinase; Gamma-glutamyl phosphate reductase; GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase 
Gene Name
 ALDH18A1 
Gene Synonyms/Alias
 GSAS; P5CS; PYCS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
149HSGQNQLKEMAIPVLubiquitination[1]
300QSVTFGTKSRVGMGGubiquitination[1, 2, 3, 4]
311GMGGMEAKVKAALWAubiquitination[1, 3]
347ITDIVEGKKVGTFFSacetylation[5]
347ITDIVEGKKVGTFFSubiquitination[1, 2]
348TDIVEGKKVGTFFSEubiquitination[1, 3]
357GTFFSEVKPAGPTVEubiquitination[1]
408DEILLANKKDLEEAEubiquitination[1]
424RLAAPLLKRLSLSTSubiquitination[3, 6, 7]
432RLSLSTSKLNSLAIGubiquitination[3]
550EDLCRLDKMIDLIIPubiquitination[3]
605TRLVRDSKCEYPAACubiquitination[3]
643MLRVEQVKIHAGPKFubiquitination[3]
662TFSPSEVKSLRTEYGubiquitination[1, 2, 6, 7, 8]
761LEGLLTTKWLLRGKDubiquitination[3]
767TKWLLRGKDHVVSDFubiquitination[1, 3]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine. 
Sequence Annotation
 NP_BIND 266 267 ATP (By similarity).
 NP_BIND 305 311 ATP (By similarity).
 REGION 1 361 Glutamate 5-kinase.
 REGION 362 795 Gamma-glutamyl phosphate reductase.
 BINDING 117 117 Substrate (By similarity).
 BINDING 223 223 Substrate (By similarity).
 BINDING 246 246 Substrate; via amide nitrogen (By  
Keyword
 3D-structure; Alternative splicing; Amino-acid biosynthesis; ATP-binding; Complete proteome; Disease mutation; Kinase; Membrane; Mental retardation; Mitochondrion; Mitochondrion inner membrane; Multifunctional enzyme; NADP; Nucleotide-binding; Oxidoreductase; Polymorphism; Proline biosynthesis; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 795 AA 
Protein Sequence
MLSQVYRCGF QPFNQHLLPW VKCTTVFRSH CIQPSVIRHV RSWSNIPFIT VPLSRTHGKS 60
FAHRSELKHA KRIVVKLGSA VVTRGDECGL ALGRLASIVE QVSVLQNQGR EMMLVTSGAV 120
AFGKQRLRHE ILLSQSVRQA LHSGQNQLKE MAIPVLEARA CAAAGQSGLM ALYEAMFTQY 180
SICAAQILVT NLDFHDEQKR RNLNGTLHEL LRMNIVPIVN TNDAVVPPAE PNSDLQGVNV 240
ISVKDNDSLA ARLAVEMKTD LLIVLSDVEG LFDSPPGSDD AKLIDIFYPG DQQSVTFGTK 300
SRVGMGGMEA KVKAALWALQ GGTSVVIANG THPKVSGHVI TDIVEGKKVG TFFSEVKPAG 360
PTVEQQGEMA RSGGRMLATL EPEQRAEIIH HLADLLTDQR DEILLANKKD LEEAEGRLAA 420
PLLKRLSLST SKLNSLAIGL RQIAASSQDS VGRVLRRTRI AKNLELEQVT VPIGVLLVIF 480
ESRPDCLPQV AALAIASGNG LLLKGGKEAA HSNRILHLLT QEALSIHGVK EAVQLVNTRE 540
EVEDLCRLDK MIDLIIPRGS SQLVRDIQKA AKGIPVMGHS EGICHMYVDS EASVDKVTRL 600
VRDSKCEYPA ACNALETLLI HRDLLRTPLF DQIIDMLRVE QVKIHAGPKF ASYLTFSPSE 660
VKSLRTEYGD LELCIEVVDN VQDAIDHIHK YGSSHTDVIV TEDENTAEFF LQHVDSACVF 720
WNASTRFSDG YRFGLGAEVG ISTSRIHARG PVGLEGLLTT KWLLRGKDHV VSDFSEHGSL 780
KYLHENLPIP QRNTN 795 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004349; F:glutamate 5-kinase activity; IDA:UniProtKB.
 GO:0004350; F:glutamate-5-semialdehyde dehydrogenase activity; IDA:UniProtKB.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0019240; P:citrulline biosynthetic process; IMP:UniProtKB.
 GO:0006536; P:glutamate metabolic process; IMP:UniProtKB.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006592; P:ornithine biosynthetic process; IMP:UniProtKB.
 GO:0006561; P:proline biosynthetic process; IMP:UniProtKB. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom.
 IPR001048; Asp/Glu/Uridylate_kinase.
 IPR000965; G-glutamylP_reductase.
 IPR020593; G-glutamylP_reductase_CS.
 IPR001057; Glu/AcGlu_kinase.
 IPR019797; Glutamate_5-kinase_CS.
 IPR005766; P5_carboxy_syn. 
Pfam
 PF00696; AA_kinase
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00902; GLUTAMATE_5_KINASE
 PS01223; PROA 
PRINTS
 PR00474; GLU5KINASE.