CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022101
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 35 
Protein Synonyms/Alias
 Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A 
Gene Name
 ARHGAP35 
Gene Synonyms/Alias
 GRF1; GRLF1; KIAA1722 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1096EPMDAVVKPRNEEENubiquitination[1]
1289IYRVSGNKSEMESLQubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor. 
Sequence Annotation
 DOMAIN 270 327 FF 1.
 DOMAIN 368 422 FF 2.
 DOMAIN 429 483 FF 3.
 DOMAIN 485 550 FF 4.
 DOMAIN 1249 1436 Rho-GAP.
 MOD_RES 308 308 Phosphotyrosine.
 MOD_RES 589 589 Phosphoserine.
 MOD_RES 773 773 Phosphoserine.
 MOD_RES 970 970 Phosphoserine.
 MOD_RES 975 975 Phosphoserine.
 MOD_RES 980 980 Phosphoserine (By similarity).
 MOD_RES 1072 1072 Phosphoserine.
 MOD_RES 1087 1087 Phosphotyrosine.
 MOD_RES 1105 1105 Phosphotyrosine; by ABL2 and PTK6.
 MOD_RES 1134 1134 Phosphoserine.
 MOD_RES 1150 1150 Phosphoserine.
 MOD_RES 1179 1179 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; DNA-binding; GTPase activation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1499 AA 
Protein Sequence
MMMARKQDVR IPTYNISVVG LSGTEKEKGQ CGIGKSCLCN RFVRPSADEF HLDHTSVLST 60
SDFGGRVVNN DHFLYWGEVS RSLEDCVECK MHIVEQTEFI DDQTFQPHRS TALQPYIKRA 120
AATKLASAEK LMYFCTDQLG LEQDFEQKQM PDGKLLVDGF LLGIDVSRGM NRNFDDQLKF 180
VSNLYNQLAK TKKPIVVVLT KCDEGVERYI RDAHTFALSK KNLQVVETSA RSNVNVDLAF 240
STLVQLIDKS RGKTKIIPYF EALKQQSQQI ATAKDKYEWL VSRIVKNHNE NWLSVSRKMQ 300
ASPEYQDYVY LEGTQKAKKL FLQHIHRLKH EHIERRRKLY LAALPLAFEA LIPNLDEIDH 360
LSCIKAKKLL ETKPEFLKWF VVLEETPWDA TSHIDNMENE RIPFDLMDTV PAEQLYEAHL 420
EKLRNERKRV EMRRAFKENL ETSPFITPGK PWEEARSFIM NEDFYQWLEE SVYMDIYGKH 480
QKQIIDKAKE EFQELLLEYS ELFYELELDA KPSKEKMGVI QDVLGEEQRF KALQKLQAER 540
DALILKHIHF VYHPTKETCP SCPACVDAKI EHLISSRFIR PSDRNQKNSL SDPNIDRINL 600
VILGKDGLAR ELANEIRALC TNDDKYVIDG KMYELSLRPI EGNVRLPVNS FQTPTFQPHG 660
CLCLYNSKES LSYVVESIEK SRESTLGRRD NHLVHLPLTL ILVNKRGDTS GETLHSLIQQ 720
GQQIASKLQC VFLDPASAGI GYGRNINEKQ ISQVLKGLLD SKRNLNLVSS TASIKDLADV 780
DLRIVMCLMC GDPFSADDIL FPVLQSQTCK SSHCGSNNSV LLELPIGLHK KRIELSVLSY 840
HSSFSIRKSR LVHGYIVFYS AKRKASLAML RAFLCEVQDI IPIQLVALTD GAVDVLDNDL 900
SREQLTEGEE IAQEIDGRFT SIPCSQPQHK LEIFHPFFKD VVEKKNIIEA THMYDNAAEA 960
CSTTEEVFNS PRAGSPLCNS NLQDSEEDIE PSYSLFREDT SLPSLSKDHS KLSMELEGND 1020
GLSFIMSNFE SKLNNKVPPP VKPKPPVHFE ITKGDLSYLD QGHRDGQRKS VSSSPWLPQD 1080
GFDPSDYAEP MDAVVKPRNE EENIYSVPHD STQGKIITIR NINKAQSNGS GNGSDSEMDT 1140
SSLERGRKVS IVSKPVLYRT RCTRLGRFAS YRTSFSVGSD DELGPIRKKE EDQASQGYKG 1200
DNAVIPYETD EDPRRRNILR SLRRNTKKPK PKPRPSITKA TWESNYFGVP LTTVVTPEKP 1260
IPIFIERCIE YIEATGLSTE GIYRVSGNKS EMESLQRQFD QDHNLDLAEK DFTVNTVAGA 1320
MKSFFSELPD PLVPYNMQID LVEAHKINDR EQKLHALKEV LKKFPKENHE VFKYVISHLN 1380
KVSHNNKVNL MTSENLSICF WPTLMRPDFS TMDALTATRT YQTIIELFIQ QCPFFFYNRP 1440
ITEPPGARPS SPSAVASTVP FLTSTPVTSQ PSPPQSPPPT PQSPMQPLLP SQLQAEHTL 1499 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IC:UniProtKB.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0005100; F:Rho GTPase activator activity; TAS:Reactome.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0043010; P:camera-type eye development; IEA:Compara.
 GO:0030900; P:forebrain development; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0043116; P:negative regulation of vascular permeability; IEA:Compara.
 GO:0001843; P:neural tube closure; IEA:Compara.
 GO:0008360; P:regulation of cell shape; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; TAS:ProtInc. 
Interpro
 IPR002713; FF_domain.
 IPR027417; P-loop_NTPase.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001806; Small_GTPase. 
Pfam
 PF01846; FF
 PF00071; Ras
 PF00620; RhoGAP 
SMART
 SM00441; FF
 SM00324; RhoGAP 
PROSITE
 PS51676; FF
 PS50238; RHOGAP 
PRINTS