| Tag | Content |
|---|
| CPLM ID | CPLM-022955 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Ubiquitin carboxyl-terminal hydrolase 25 |
Protein Synonyms/Alias | Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25 |
Gene Name | USP25 |
Gene Synonyms/Alias | USP21 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 60 | FLTAKNAKTPQQEET | ubiquitination | [1] | | 201 | RRLVLNYKPPSNAQD | ubiquitination | [1] | | 254 | EILKDAFKSNDSQQQ | ubiquitination | [1] | | 584 | IELMYSDKSMIQVPY | ubiquitination | [1] | | 630 | YNDIAVTKSSWEELV | ubiquitination | [1] | | 710 | WDAQLAQKALQEKLL | ubiquitination | [1] | | 905 | EWHQDYRKFRETTMY | ubiquitination | [1] |
|
Reference | [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization. Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW. Nature. 2013 Apr 18;496(7445):372-6. [ PMID: 23503661] |
Functional Description | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. |
Sequence Annotation | DOMAIN 14 57 UBA-like.
REPEAT 97 116 UIM 1.
REPEAT 123 140 UIM 2.
REGION 77 102 SUMO interaction domain (SIM).
MOTIF 89 95 Required for SUMO paralog-specific
ACT_SITE 178 178
ACT_SITE 599 599 By similarity.
ACT_SITE 607 607 By similarity.
CROSSLNK 99 99 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 99 99 Glycyl lysine isopeptide (Lys-Gly) |
Keyword | Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Hydrolase; Isopeptide bond; Nucleus; Phosphoprotein; Protease; Reference proteome; Repeat; Thiol protease; Ubl conjugation; Ubl conjugation pathway. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1055 AA |
Protein Sequence | MTVEQNVLQQ SAAQKHQQTF LNQLREITGI NDTQILQQAL KDSNGNLELA VAFLTAKNAK 60
TPQQEETTYY QTALPGNDRY ISVGSQADTN VIDLTGDDKD DLQRAIALSL AESNRAFRET 120
GITDEEQAIS RVLEASIAEN KACLKRTPTE VWRDSRNPYD RKRQDKAPVG LKNVGNTCWF 180
SAVIQSLFNL LEFRRLVLNY KPPSNAQDLP RNQKEHRNLP FMRELRYLFA LLVGTKRKYV 240
DPSRAVEILK DAFKSNDSQQ QDVSEFTHKL LDWLEDAFQM KAEEETDEEK PKNPMVELFY 300
GRFLAVGVLE GKKFENTEMF GQYPLQVNGF KDLHECLEAA MIEGEIESLH SENSGKSGQE 360
HWFTELPPVL TFELSRFEFN QALGRPEKIH NKLEFPQVLY LDRYMHRNRE ITRIKREEIK 420
RLKDYLTVLQ QRLERYLSYG SGPKRFPLVD VLQYALEFAS SKPVCTSPVD DIDASSPPSG 480
SIPSQTLPST TEQQGALSSE LPSTSPSSVA AISSRSVIHK PFTQSRIPPD LPMHPAPRHI 540
TEEELSVLES CLHRWRTEIE NDTRDLQESI SRIHRTIELM YSDKSMIQVP YRLHAVLVHE 600
GQANAGHYWA YIFDHRESRW MKYNDIAVTK SSWEELVRDS FGGYRNASAY CLMYINDKAQ 660
FLIQEEFNKE TGQPLVGIET LPPDLRDFVE EDNQRFEKEL EEWDAQLAQK ALQEKLLASQ 720
KLRESETSVT TAQAAGDPEY LEQPSRSDFS KHLKEETIQI ITKASHEHED KSPETVLQSI 780
MMTPNMQGII MAIGKSRSVY DRCGPEAGFF KAIKLEYARL VKLAQEDTPP ETDYRLHHVV 840
VYFIQNQAPK KIIEKTLLEQ FGDRNLSFDE RCHNIMKVAQ AKLEMIKPEE VNLEEYEEWH 900
QDYRKFRETT MYLIIGLENF QRESYIDSLL FLICAYQNNK ELLSKGLYRG HDEELISHYR 960
RECLLKLNEQ AAELFESGED REVNNGLIIM NEFIVPFLPL LLVDEMEEKD ILAVEDMRNR 1020
WCSYLGQEME PHLQEKLTDF LPKLLDCSME IKSFHEPPKL PSYSTHELCE RFARIMLSLS 1080
RTPADGR 1087 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. GO:0032183; F:SUMO binding; IDA:UniProtKB. GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro. GO:0004843; F:ubiquitin-specific protease activity; IMP:UniProtKB. GO:0071108; P:protein K48-linked deubiquitination; IMP:UniProtKB. GO:0070536; P:protein K63-linked deubiquitination; IMP:UniProtKB. GO:0006508; P:proteolysis; TAS:ProtInc. GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |