UniProt Accession

 HSP60_YEAST; P19882; D6VYQ6 

Genbank Protein ID

 M33301; U17244; BK006945 

Genbank Nucleotide ID

 AAA34690.1; AAB67380.1; DAA09572.1 

Protein Name

 Heat shock protein 60, mitochondrial 

Protein Synonyms/Alias

 CPN60; P66; Stimulator factor I 66 kDa component 

Gene Name

 HSP60 

Gene Synonyms/Alias

 MIF4; YLR259C; L8479.10 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
28	YSSHKELKFGVEGRA	acetylation	[1]
69	EQPFGPPKITKDGVT	acetylation	[1]
93	KFENMGAKLLQEVAS	acetylation	[1]
147	GSQVAVEKVIEFLSA	acetylation	[1]
189	LLASAMEKVGKEGVI	acetylation	[1]
230	PYFITDPKSSKVEFE	acetylation	[1]
233	ITDPKSSKVEFEKPL	acetylation	[1]
238	SSKVEFEKPLLLLSE	acetylation	[1]
246	PLLLLSEKKISSIQD	acetylation	[1]
360	ILNGSGPKEAIQERI	acetylation	[1]
384	TTTNSYEKEKLQERL	acetylation	[1]
393	KLQERLAKLSGGVAV	acetylation	[1]
414	SEVEVGEKKDRYDDA	acetylation	[1]
414	SEVEVGEKKDRYDDA	ubiquitination	[2, 3]
501	EYGDDFAKGYDASKS	acetylation	[1]
507	AKGYDASKSEYTDML	acetylation	[1]
523	TGIIDPFKVVRSGLV	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 May participate in assembly and/or disassembly of proteins imported into the mitochondrion. HSP60 are ATPases and have affinity for unfolded proteins. 

Sequence Annotation

 MOD_RES 102 102 Phosphothreonine.
 MOD_RES 485 485 Phosphoserine.  

Keyword

 ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 572 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
 GO:0005758; C:mitochondrial intermembrane space; TAS:Reactome.
 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0003688; F:DNA replication origin binding; IDA:SGD.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0051082; F:unfolded protein binding; IMP:SGD.
 GO:0006458; P:'de novo' protein folding; IMP:SGD.
 GO:0051131; P:chaperone-mediated protein complex assembly; IMP:SGD.
 GO:0045041; P:protein import into mitochondrial intermembrane space; IMP:SGD.
 GO:0051604; P:protein maturation; IMP:SGD.
 GO:0042026; P:protein refolding; IDA:SGD.
 GO:0050821; P:protein stabilization; IMP:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR018370; Chaperonin_Cpn60_CS.
 IPR001844; Chaprnin_Cpn60.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

 PS00296; CHAPERONINS_CPN60 

PRINTS

 PR00298; CHAPERONIN60.