CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012516
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inorganic pyrophosphatase 
Protein Synonyms/Alias
 Pyrophosphate phospho-hydrolase; PPase 
Gene Name
 PPA1 
Gene Synonyms/Alias
 IOPPP; PP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22LEYRVFLKNEKGQYIubiquitination[1, 2]
25RVFLKNEKGQYISPFubiquitination[1, 3, 4, 5, 6]
57VPRWSNAKMEIATKDacetylation[2, 7, 8, 9]
63AKMEIATKDPLNPIKacetylation[2]
63AKMEIATKDPLNPIKubiquitination[1]
70KDPLNPIKQDVKKGKubiquitination[1, 3, 6]
109EDPGHNDKHTGCCGDacetylation[10]
109EDPGHNDKHTGCCGDubiquitination[1]
128DVCEIGSKVCARGEIacetylation[10]
128DVCEIGSKVCARGEIubiquitination[1]
176YNDINDVKRLKPGYLacetylation[9]
176YNDINDVKRLKPGYLubiquitination[1, 2, 3, 5, 6, 11, 12, 13]
179INDVKRLKPGYLEATubiquitination[1, 3, 6]
194VDWFRRYKVPDGKPEacetylation[2]
199RYKVPDGKPENEFAFacetylation[2]
221DFAIDIIKSTHDHWKubiquitination[3, 6]
228KSTHDHWKALVTKKTacetylation[2, 7, 8, 9]
228KSTHDHWKALVTKKTubiquitination[1, 3, 4, 6, 12]
238VTKKTNGKGISCMNTubiquitination[1, 4]
253TLSESPFKCDPDAARubiquitination[13]
282TVPTDVDKWFHHQKNubiquitination[1]
288DKWFHHQKN******ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [11] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [12] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [13] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
 METAL 116 116 Magnesium 1 (By similarity).
 METAL 121 121 Magnesium 1 (By similarity).
 METAL 121 121 Magnesium 2 (By similarity).
 METAL 153 153 Magnesium 1 (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 57 57 N6-acetyllysine.
 MOD_RES 228 228 N6-acetyllysine.
 MOD_RES 250 250 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 289 AA 
Protein Sequence
MSGFSTEERA APFSLEYRVF LKNEKGQYIS PFHDIPIYAD KDVFHMVVEV PRWSNAKMEI 60
ATKDPLNPIK QDVKKGKLRY VANLFPYKGY IWNYGAIPQT WEDPGHNDKH TGCCGDNDPI 120
DVCEIGSKVC ARGEIIGVKV LGILAMIDEG ETDWKVIAIN VDDPDAANYN DINDVKRLKP 180
GYLEATVDWF RRYKVPDGKP ENEFAFNAEF KDKDFAIDII KSTHDHWKAL VTKKTNGKGI 240
SCMNTTLSES PFKCDPDAAR AIVDALPPPC ESACTVPTDV DKWFHHQKN 289 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004427; F:inorganic diphosphatase activity; EXP:Reactome.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0071344; P:diphosphate metabolic process; TAS:Reactome.
 GO:0006796; P:phosphate-containing compound metabolic process; TAS:ProtInc.
 GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome. 
Interpro
 IPR008162; Pyrophosphatase. 
Pfam
 PF00719; Pyrophosphatase 
SMART
  
PROSITE
 PS00387; PPASE 
PRINTS