CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005157
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Casein kinase I homolog 2 
Protein Synonyms/Alias
  
Gene Name
 YCK2 
Gene Synonyms/Alias
 CKI1; YNL154C; N1755 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
286QLPWQGLKAPNNKQKubiquitination[1]
465DEEREASKLDPTSYEubiquitination[1, 2]
481YQQQTQQKYAQQQQKubiquitination[1, 2]
488KYAQQQQKQMQQKSKubiquitination[1]
495KQMQQKSKQFANTGAubiquitination[1]
508GANGQTNKYPYNAQPubiquitination[1, 2]
524ANDEQNAKNAAQDRNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. 
Sequence Annotation
 DOMAIN 76 360 Protein kinase.
 NP_BIND 82 90 ATP (By similarity).
 ACT_SITE 195 195 Proton acceptor (By similarity).
 BINDING 105 105 ATP (By similarity).
 MOD_RES 455 455 Phosphoserine.
 LIPID 545 545 S-palmitoyl cysteine.
 LIPID 546 546 S-palmitoyl cysteine.  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Kinase; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 546 AA 
Protein Sequence
MSQVQSPLTA TNSGLAVNNN TMNSQMPNRS NVRLVNGTLP PSLHVSSNLN HNTGNSSASY 60
SGSQSRDDST IVGLHYKIGK KIGEGSFGVL FEGTNMINGL PVAIKFEPRK TEAPQLKDEY 120
RTYKILAGTP GIPQEYYFGQ EGLHNILVID LLGPSLEDLF DWCGRRFSVK TVVQVAVQMI 180
TLIEDLHAHD LIYRDIKPDN FLIGRPGQPD ANKVHLIDFG MAKQYRDPKT KQHIPYREKK 240
SLSGTARYMS INTHLGREQS RRDDMEAMGH VFFYFLRGQL PWQGLKAPNN KQKYEKIGEK 300
KRLTNVYDLA QGLPIQFGRY LEIVRNLSFE ETPDYEGYRM LLLSVLDDLG ETADGQYDWM 360
KLNGGRGWDL SINKKPNLHG YGHPNPPNEK SKRHRSKNHQ YSSPDHHHHY NQQQQQQQAQ 420
AQAQAQAQAK VQQQQLQQAQ AQQQANRYQL QPDDSHYDEE REASKLDPTS YEAYQQQTQQ 480
KYAQQQQKQM QQKSKQFANT GANGQTNKYP YNAQPTANDE QNAKNAAQDR NSNKSSKGFF 540
SKLGCC 546 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005937; C:mating projection; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0000902; P:cell morphogenesis; IGI:SGD.
 GO:0000910; P:cytokinesis; IGI:SGD.
 GO:0006897; P:endocytosis; IGI:SGD.
 GO:0009749; P:response to glucose stimulus; IMP:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS