CPLM-000241

Genbank Nucleotide ID

TRAF-type zinc finger domain-containing protein 1

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
43	IGMCPTCKEPFPKSD	ubiquitination	[1]
65	EHCQVTCKCNKKLEK	ubiquitination	[1]
77	LEKRLLKKHEETECP	ubiquitination	[1]
103	ELSILKLKEHEDYCG	ubiquitination	[1, 2, 3]
126	CGRNVLVKDLKTHPE	ubiquitination	[1]
129	NVLVKDLKTHPEVCG	ubiquitination	[1, 2]
142	CGREGEEKRNEVAIP	ubiquitination	[2]
283	PRSLSDIKGAADEIM	ubiquitination	[3]
485	PSSPCVPKLSNSDSQ	ubiquitination	[4, 5, 6]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3 (By similarity).

ZN_FING 27 103 TRAF-type.
MOD_RES 326 326 Phosphoserine.
MOD_RES 327 327 Phosphoserine.
MOD_RES 415 415 Phosphoserine.
MOD_RES 470 470 Phosphoserine.

3D-structure; Complete proteome; Metal-binding; Phosphoprotein; Reference proteome; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0045824; P:negative regulation of innate immune response; ISS:UniProtKB.
GO:0034097; P:response to cytokine stimulus; IEA:Compara.

IPR015880; Znf_C2H2-like.