UniProt Accession

 C9J2Y9_HUMAN; C9J2Y9 

Genbank Protein ID

 AC069307 

Genbank Nucleotide ID

  

Protein Name

 DNA-directed RNA polymerase 

Protein Synonyms/Alias

  

Gene Name

 POLR2B 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
80	EPPRYLLKFEQIYLS	ubiquitination	[1, 2, 3]
88	FEQIYLSKPTHWERD	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
123	PLYVDITKTVIKEGE	ubiquitination	[1, 2, 3, 5, 7]
127	DITKTVIKEGEEQLQ	ubiquitination	[1, 2, 3, 5, 7]
139	QLQTQHQKTFIGKIP	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
144	HQKTFIGKIPIMLRS	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
192	KVLIAQEKMATNTVY	ubiquitination	[1, 2, 7]
203	NTVYVFAKKDSKYAY	ubiquitination	[1, 2, 7]
204	TVYVFAKKDSKYAYT	ubiquitination	[2, 3, 5]
207	VFAKKDSKYAYTGEC	ubiquitination	[2, 3, 5]
241	ARGGQGAKKSAIGQR	ubiquitination	[1, 7]
242	RGGQGAKKSAIGQRI	ubiquitination	[1, 7]
257	VATLPYIKQEVPIII	ubiquitination	[1, 2, 3, 5, 7]
330	VTKEKRIKYAKEVLQ	ubiquitination	[3]
333	EKRIKYAKEVLQKEM	ubiquitination	[1, 2, 3, 7]
338	YAKEVLQKEMLPHVG	ubiquitination	[2, 3]
353	VSDFCETKKAYFLGY	ubiquitination	[2, 5]
354	SDFCETKKAYFLGYM	ubiquitination	[2, 3]
384	DRDHYGNKRLDLAGP	ubiquitination	[1, 2, 3, 5, 7]
406	GMFKNLLKEVRIYAQ	ubiquitination	[1]
414	EVRIYAQKFIDRGKD	ubiquitination	[1, 2, 4]
420	QKFIDRGKDFNLELA	ubiquitination	[1, 2, 3, 5]
429	FNLELAIKTRIISDG	ubiquitination	[1, 5]
438	RIISDGLKYSLATGN	ubiquitination	[1, 2, 3, 7]
450	TGNWGDQKKAHQARA	ubiquitination	[1, 2, 3, 5, 6, 7]
451	GNWGDQKKAHQARAG	ubiquitination	[2, 3]
571	GCWVGIHKDPEQLMN	ubiquitination	[2, 3, 5]
623	RPLLIVEKQKLLLKK	ubiquitination	[1, 2, 3, 6, 7]
625	LLIVEKQKLLLKKRH	ubiquitination	[2, 3]
637	KRHIDQLKEREYNNY	ubiquitination	[1, 2, 5, 6, 7]
723	TYQSAMGKQAMGVYI	ubiquitination	[1, 2, 3, 5, 6, 7]
749	HVLYYPQKPLVTTRS	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
808	SVFYRSYKEQESKKG	ubiquitination	[1]
814	YKEQESKKGFDQEEV	ubiquitination	[1, 2, 3, 5, 7]
824	DQEEVFEKPTRETCQ	ubiquitination	[1, 2, 3, 5, 6, 7]
840	MRHAIYDKLDDDGLI	ubiquitination	[1, 2, 3, 4, 5, 7]
862	GDDVIIGKTVTLPEN	ubiquitination	[1, 2, 3, 5, 6, 7]
910	TLNQEGYKFCKIRVR	ubiquitination	[2, 3, 5]
927	RIPQIGDKFASRHGQ	ubiquitination	[1, 2, 3, 4, 6, 7]
935	FASRHGQKGTCGIQY	ubiquitination	[2, 3, 5]
981	LIECLQGKVSANKGE	ubiquitination	[2, 3, 5]
986	QGKVSANKGEIGDAT	ubiquitination	[1, 2, 3, 5, 6, 7]
1003	NDAVNVQKISNLLSD	ubiquitination	[1, 2, 3, 5, 7]
1029	YNGFTGRKITSQIFI	ubiquitination	[1, 2, 3, 6]
1045	PTYYQRLKHMVDDKI	methylation	[8]
1051	LKHMVDDKIHSRARG	ubiquitination	[1]
1136	ECRGCRNKTQISLVR	ubiquitination	[1, 2, 3, 6, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome; Transcription; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1167 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0032549; F:ribonucleoside binding; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:InterPro. 

Interpro

 IPR015712; DNA-dir_RNA_pol_su2.
 IPR007120; DNA-dir_RNA_pol_su2_6.
 IPR007121; RNA_pol_bsu_CS.
 IPR007644; RNA_pol_bsu_protrusion.
 IPR007642; RNA_pol_Rpb2_2.
 IPR007645; RNA_pol_Rpb2_3.
 IPR007646; RNA_pol_Rpb2_4.
 IPR007647; RNA_pol_Rpb2_5.
 IPR007641; RNA_pol_Rpb2_7.
 IPR014724; RNA_pol_RPB2_OB-fold. 

Pfam

 PF04563; RNA_pol_Rpb2_1
 PF04561; RNA_pol_Rpb2_2
 PF04565; RNA_pol_Rpb2_3
 PF04566; RNA_pol_Rpb2_4
 PF04567; RNA_pol_Rpb2_5
 PF00562; RNA_pol_Rpb2_6
 PF04560; RNA_pol_Rpb2_7 

SMART

  

PROSITE

 PS01166; RNA_POL_BETA 

PRINTS