CPLM-001349

Genbank Nucleotide ID

WD repeat-containing protein 1

Protein Synonyms/Alias

Actin-interacting protein 1; AIP1

Mus musculus (Mouse)

Position	Peptide	Type	References
7	*MPYEIKKVFASLPQ	acetylation	[1, 2]
7	*MPYEIKKVFASLPQ	succinylation	[2]
7	*MPYEIKKVFASLPQ	ubiquitination	[3]
21	QVERGVSKILGGDPK	acetylation	[1, 2]
21	QVERGVSKILGGDPK	succinylation	[2]
28	KILGGDPKGDHFLYT	acetylation	[4]
38	HFLYTNGKCVILRNI	ubiquitination	[3]
81	ASGDISGKLRIWDTT	ubiquitination	[3]
90	RIWDTTQKEHLLKYE	acetylation	[2]
90	RIWDTTQKEHLLKYE	succinylation	[2]
95	TQKEHLLKYEYQPFA	acetylation	[1, 2, 4]
95	TQKEHLLKYEYQPFA	ubiquitination	[3]
104	EYQPFAGKIKDIAWT	acetylation	[2, 4]
104	EYQPFAGKIKDIAWT	succinylation	[2]
104	EYQPFAGKIKDIAWT	ubiquitination	[3]
106	QPFAGKIKDIAWTED	ubiquitination	[3]
180	FFEGPPFKFKFTIGD	acetylation	[5]
182	EGPPFKFKFTIGDHS	acetylation	[1]
219	QIFIYDGKTGEKVCA	acetylation	[4]
223	YDGKTGEKVCALGES	acetylation	[2]
223	YDGKTGEKVCALGES	succinylation	[2]
256	LLSASGDKTSKIWDV	acetylation	[2, 4]
256	LLSASGDKTSKIWDV	ubiquitination	[3]
321	RVIKGHSKSIQCLTV	acetylation	[1]
405	YSGQGVVKLDVQPKC	ubiquitination	[3]
480	SILASTLKDEGKLLE	acetylation	[1, 2]
480	SILASTLKDEGKLLE	ubiquitination	[3]

[1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]

Functional Description

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (By similarity).

REPEAT 56 95 WD 1.
REPEAT 100 143 WD 2.
REPEAT 144 184 WD 3.
REPEAT 187 226 WD 4.
REPEAT 232 271 WD 5.
REPEAT 318 359 WD 6.
REPEAT 363 401 WD 7.
REPEAT 443 482 WD 8.
REPEAT 487 526 WD 9.
REPEAT 530 569 WD 10.
REPEAT 574 605 WD 11.
MOD_RES 28 28 N6-acetyllysine (By similarity).
MOD_RES 81 81 N6-acetyllysine (By similarity).
MOD_RES 95 95 N6-acetyllysine (By similarity).
MOD_RES 115 115 N6-acetyllysine (By similarity).
MOD_RES 238 238 Phosphotyrosine.
MOD_RES 480 480 N6-acetyllysine (By similarity).

Acetylation; Actin-binding; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0015629; C:actin cytoskeleton; ISA:MGI.
GO:0030054; C:cell junction; IEA:UniProtKB-KW.
GO:0042995; C:cell projection; IEA:UniProtKB-KW.
GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
GO:0003779; F:actin binding; ISA:MGI.
GO:0007605; P:sensory perception of sound; ISA:MGI.

IPR020472; G-protein_beta_WD-40_rep.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00320; GPROTEINBRPT.