CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019578
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleus accumbens-associated protein 1 
Protein Synonyms/Alias
 NAC-1; BTB/POZ domain-containing protein 14B 
Gene Name
 NACC1 
Gene Synonyms/Alias
 BTBD14B; NAC1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
167LPLVSRVKTEQQESDubiquitination[1]
191RLWDSGQKEAGGGGNubiquitination[2]
368DEGDPSEKLELVTGTubiquitination[3]
452QNFAPNFKESEMNAIubiquitination[2, 4]
483MPKVKVLKAEDDAYTubiquitination[3]
498TFISETGKIEPDMMGubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines. 
Sequence Annotation
 DOMAIN 30 94 BTB.
 DOMAIN 374 471 BEN.
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 259 259 Phosphoserine; by PKC (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MAQTLQMEIP NFGNSILECL NEQRLQGLYC DVSVVVKGHA FKAHRAVLAA SSSYFRDLFN 60
NSRSAVVELP AAVQPQSFQQ ILSFCYTGRL SMNVGDQFLL MYTAGFLQIQ EIMEKGTEFF 120
LKVSSPSCDS QGLHAEEAPS SEPQSPVAQT SGWPACSTPL PLVSRVKTEQ QESDSVQCMP 180
VAKRLWDSGQ KEAGGGGNGS RKMAKFSTPD LAANRPHQPP PPQQAPVVAA AQPAVAAGAG 240
QPAGGVAAAG GVVSGPSTSE RTSPGTSSAY TSDSPGSYHN EEDEEEDGGE EGMDEQYRQI 300
CNMYTMYSMM NVGQTAEKVE ALPEQVAPES RNRIRVRQDL ASLPAELINQ IGNRCHPKLY 360
DEGDPSEKLE LVTGTNVYIT RAQLMNCHVS AGTRHKVLLR RLLASFFDRN TLANSCGTGI 420
RSSTNDPRRK PLDSRVLHAV KYYCQNFAPN FKESEMNAIA ADMCTNARRV VRKSWMPKVK 480
VLKAEDDAYT TFISETGKIE PDMMGVEHGF ETASHEGEAG PSAEALQ 527 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016604; C:nuclear body; IDA:UniProtKB.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
 GO:0051260; P:protein homooligomerization; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR018379; BEN_domain.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ. 
Pfam
 PF10523; BEN
 PF00651; BTB 
SMART
 SM01025; BEN
 SM00225; BTB 
PROSITE
 PS51457; BEN
 PS50097; BTB 
PRINTS