CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029792
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nardilysin 
Protein Synonyms/Alias
 Nardilysin (N-arginine dibasic convertase), isoform CRA_d 
Gene Name
 NRD1 
Gene Synonyms/Alias
 hCG_40292 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
209ARKKTTEKQQLQSLFubiquitination[1]
229LTDRLWFKSTYSKMSubiquitination[2, 3, 4]
234WFKSTYSKMSSTLLVubiquitination[1]
325NGFDAFLKKHGGSDNubiquitination[1]
326GFDAFLKKHGGSDNAubiquitination[5]
352DVQRKYFKEALDRWAubiquitination[2, 4, 5]
394RPSDANRKEMLFGSLubiquitination[2, 3, 4, 5, 6]
410RPGHPMGKFFWGNAEubiquitination[2, 4]
420WGNAETLKHEPRKNNubiquitination[2, 4]
602QYLKMLQKLGPEKRIubiquitination[7]
763QGCLWYKKDNKFKIPubiquitination[5]
981CKVKALNKGDANSEVubiquitination[1]
1023CFDFLRTKQTLGYHVubiquitination[6]
1061YNSEVVDKKIEEFLSacetylation[6]
1061YNSEVVDKKIEEFLSubiquitination[6]
1129AHEIEALKSFSKSDLubiquitination[1]
1133EALKSFSKSDLVNWFubiquitination[1, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteomic snapshot of the EGF-induced ubiquitin network.
 Argenzio E, Bange T, Oldrini B, Bianchi F, Peesari R, Mari S, Di Fiore PP, Mann M, Polo S.
 Mol Syst Biol. 2011 Jan 18;7:462. [PMID: 21245847
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1219 AA 
Protein Sequence
MLRRVTVAAV CATRRKLCEA GRELAALWGI ETRGRCEDSA AARPFPILAM PGRNKAKSTC 60
SCPDLQPNGQ DLGENSRVAR LGADESEEEG RRGSLSNAGD PEIVKSPSDP KQYRYIKLQN 120
GLQALLISDL SNMEGKTGNT TDDEEEEEVE EEEEDDDEDS GAEIEDDDEE GFDDEDEFDD 180
EHDDDLDTED NELEELEERA EARKKTTEKQ QLQSLFLLWS KLTDRLWFKS TYSKMSSTLL 240
VETRNLYGVV GAESRSAPVQ HLAGWQAEEQ QGETDTVLSA AALCVGVGSF ADPDDLPGLA 300
HFLEHMVFMG SLKYPDENGF DAFLKKHGGS DNASTDCERT VFQFDVQRKY FKEALDRWAQ 360
FFIHPLMIRD AIDREVEAVD SEYQLARPSD ANRKEMLFGS LARPGHPMGK FFWGNAETLK 420
HEPRKNNIDT HARLREFWMR YYSSHYMTLV VQSKETLDTL EKWVTEIFSQ IPNNGLPRPN 480
FGHLTDPFDT PAFNKLYRVV PIRKIHALTI TWALPPQQQH YRVKPLHYIS WLVGHEGKGS 540
ILSFLRKKCW ALALFGGNGE TGFEQNSTYS VFSISITLTD EGYEHFYEVA YTVFQYLKML 600
QKLGPEKRIF EEIRKIEDNE FHYQEQTDPV EYVENMCENM QLYPLQDILT GDQLLFEYKP 660
EVIGEALNQL VPQKANLVLL SGANEGKCDL KEKWFGTQYS IEDIENSWAE LWNSNFELNP 720
DLHLPAENKY IATDFTLKAF DCPETEYPVK IVNTPQGCLW YKKDNKFKIP KAYIRFHLIS 780
PLIQKSAANV VLFDIFVNIL THNLAEPAYE ADVAQLEYKL VAGEHGLIIR VKGFNHKLPL 840
LFQLIIDYLA EFNSTPAVFT MITEQLKKTY FNILIKPETL AKDVRLLILE YARWSMIDKY 900
QALMDGLSLE SLLSFVKEFK SQLFVEGLVQ GNVTSTESMD FLKYVVDKLN FKPLEQEMPV 960
QFQVVELPSG HHLCKVKALN KGDANSEVTV YYQSGTRSLR EYTLMELLVM HMEEPCFDFL 1020
RTKQTLGYHV YPTCRNTSGI LGFSVTVGTQ ATKYNSEVVD KKIEEFLSSF EEKIENLTEE 1080
AFNTQVTALI KLKECEDTHL GEEVDRNWNE VVTQQYLFDR LAHEIEALKS FSKSDLVNWF 1140
KAHRGPGSKM LSVHVVGYGK YELEEDGTPS SEDSNSSCEV MQLTYLPTSP LLADCIIPIT 1200
DIRAFTTTLN LLPYHKIVK 1219 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:InterPro.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:InterPro. 
Interpro
 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 
Pfam
 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 
SMART
  
PROSITE
 PS00143; INSULINASE 
PRINTS