CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008969
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Caspase-3 
Protein Synonyms/Alias
 CASP-3; Apopain; Cysteine protease CPP32; CPP-32; IRP; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1; Caspase-3 subunit p17; Caspase-3 subunit p12 
Gene Name
 Casp3 
Gene Synonyms/Alias
 Cpp32 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
11NETSVDSKSINNFETacetylation[1]
25TKTIHGSKSMDSGIYacetylation[1]
229MLKLYAHKLEFMHILacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). 
Sequence Annotation
 ACT_SITE 121 121 By similarity.
 ACT_SITE 163 163 By similarity.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 163 163 S-nitrosocysteine; in inhibited form (By  
Keyword
 Acetylation; Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; S-nitrosylation; Thiol protease; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 277 AA 
Protein Sequence
MDNNETSVDS KSINNFETKT IHGSKSMDSG IYLDSSYKMD YPEMGLCIII NNKNFHKSTG 60
MSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS 120
HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETDSGTDD 180
DMACQKIPVE ADFLYAYSTA PGYYSWRNSR DGSWFIQSLC AMLKLYAHKL EFMHILTRVN 240
RKVATEFESF SLDATFHAKK QIPCIVSMLT KELYFYH 277 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
 GO:0008234; F:cysteine-type peptidase activity; IDA:RGD.
 GO:0016005; F:phospholipase A2 activator activity; IMP:RGD.
 GO:0071407; P:cellular response to organic cyclic compound; IEP:RGD.
 GO:0034349; P:glial cell apoptotic process; IEP:RGD.
 GO:0021766; P:hippocampus development; IEP:RGD.
 GO:0006917; P:induction of apoptosis; TAS:RGD.
 GO:0035556; P:intracellular signal transduction; IMP:RGD.
 GO:0007611; P:learning or memory; IMP:RGD.
 GO:0030182; P:neuron differentiation; IEP:RGD.
 GO:0043525; P:positive regulation of neuron apoptotic process; IMP:RGD.
 GO:0006508; P:proteolysis; IDA:RGD.
 GO:0043200; P:response to amino acid stimulus; IEP:RGD.
 GO:0046677; P:response to antibiotic; IEP:RGD.
 GO:0032025; P:response to cobalt ion; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032355; P:response to estradiol stimulus; IEP:RGD.
 GO:0051384; P:response to glucocorticoid stimulus; IEP:RGD.
 GO:0009749; P:response to glucose stimulus; IDA:RGD.
 GO:0042542; P:response to hydrogen peroxide; IDA:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
 GO:0035094; P:response to nicotine; IEP:RGD.
 GO:0010165; P:response to X-ray; IEP:RGD.
 GO:0042060; P:wound healing; IEP:RGD. 
Interpro
 IPR015470; Caspase_3.
 IPR011600; Pept_C14_cat.
 IPR001309; Pept_C14_ICE_p20.
 IPR016129; Pept_C14_ICE_p20_AS.
 IPR002138; Pept_C14_p10.
 IPR002398; Pept_C14_p45.
 IPR015917; Pept_C14_p45_core. 
Pfam
 PF00656; Peptidase_C14 
SMART
 SM00115; CASc 
PROSITE
 PS01122; CASPASE_CYS
 PS01121; CASPASE_HIS
 PS50207; CASPASE_P10
 PS50208; CASPASE_P20 
PRINTS
 PR00376; IL1BCENZYME.