UniProt Accession

 T2FB_HUMAN; P13984; A6NNS5; Q5W0H3 

Genbank Protein ID

 X16901; X59745; AK291545; BT019525; AL138963; AL138693; AL138693; AL138963; CH471075; BC001771 

Genbank Nucleotide ID

 CAA34775.1; CAA42419.1; BAF84234.1; AAV38332.1; CAH72032.1; CAH72032.1; CAH72459.1; CAH72459.1; EAX08728.1; AAH01771.1 

Protein Name

 General transcription factor IIF subunit 2 

Protein Synonyms/Alias

 ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30 

Gene Name

 GTF2F2 

Gene Synonyms/Alias

 RAP30 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
13	ELDLTGAKQNTGVWL	ubiquitination	[1, 2]
22	NTGVWLVKVPKYLSQ	acetylation	[3]
22	NTGVWLVKVPKYLSQ	ubiquitination	[2, 4]
25	VWLVKVPKYLSQQWA	ubiquitination	[2]
33	YLSQQWAKASGRGEV	acetylation	[3]
33	YLSQQWAKASGRGEV	ubiquitination	[2]
42	SGRGEVGKLRIAKTQ	acetylation	[4]
71	NIHDIGGKPASVSAP	acetylation	[4]
71	NIHDIGGKPASVSAP	ubiquitination	[1]
137	LQIEESSKPVRLSQQ	acetylation	[3, 4, 5]
137	LQIEESSKPVRLSQQ	ubiquitination	[1, 2, 4]
147	RLSQQLDKVVTTNYK	ubiquitination	[6, 7]
154	KVVTTNYKPVANHQY	acetylation	[4, 5, 8]
154	KVVTTNYKPVANHQY	ubiquitination	[2, 6, 7, 9]
193	MLFSAFEKHQYYNLK	acetylation	[10]
200	KHQYYNLKDLVDITK	ubiquitination	[6, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [10] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224] 

Functional Description

 TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. 

Sequence Annotation

 NP_BIND 36 43 ATP (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 22 22 N6-acetyllysine.
 MOD_RES 33 33 N6-acetyllysine.
 MOD_RES 137 137 N6-acetyllysine.
 MOD_RES 142 142 Phosphoserine.
 MOD_RES 248 248 Phosphoserine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Direct protein sequencing; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 249 AA 

Protein Sequence

Gene Ontology

 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005674; C:transcription factor TFIIF complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR016640; TFIIF-beta_subgr.
 IPR003196; TFIIF_beta.
 IPR011039; TFIIF_interaction.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02270; TFIIF_beta 

SMART

  

PROSITE

  

PRINTS