UniProt Accession

 3HIDH_MOUSE; Q99L13; Q8BJY2 

Genbank Protein ID

 BC003914; AK078175 

Genbank Nucleotide ID

 AAH03914.1; BAC37162.1 

Protein Name

 3-hydroxyisobutyrate dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 HIBADH 

Gene Name

 Hibadh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
55	NMGNPMAKNLMKHGY	acetylation	[1]
55	NMGNPMAKNLMKHGY	ubiquitination	[2]
59	PMAKNLMKHGYPLIL	acetylation	[1, 3, 4, 5]
59	PMAKNLMKHGYPLIL	succinylation	[5]
75	DVFPDVCKEFKEAGE	acetylation	[1, 3, 4, 5]
75	DVFPDVCKEFKEAGE	succinylation	[5]
78	PDVCKEFKEAGEQVA	acetylation	[1, 3, 4, 5, 6, 7, 8]
78	PDVCKEFKEAGEQVA	succinylation	[5]
94	SPAEVAEKADRIITM	acetylation	[1, 3, 4, 5, 6]
94	SPAEVAEKADRIITM	succinylation	[5]
120	SGANGILKKVKKGSL	acetylation	[1, 4]
140	TIDPSVSKELAKEVE	acetylation	[1, 3, 4, 5, 6]
140	TIDPSVSKELAKEVE	succinylation	[5]
144	SVSKELAKEVEKMGA	acetylation	[1, 3, 4, 9]
148	ELAKEVEKMGAVFMD	acetylation	[1, 3, 4, 5, 6]
148	ELAKEVEKMGAVFMD	succinylation	[5]
237	IRSGLDPKLLAKILN	acetylation	[1, 3, 4, 5, 6, 8, 10]
237	IRSGLDPKLLAKILN	succinylation	[5]
241	LDPKLLAKILNMSSG	acetylation	[3, 4, 5, 7, 9, 11]
241	LDPKLLAKILNMSSG	succinylation	[5]
241	LDPKLLAKILNMSSG	ubiquitination	[2]
296	QDSATSTKTPILLGS	acetylation	[1, 3, 5]
296	QDSATSTKTPILLGS	succinylation	[5]
320	CSKGYSKKDFSSVFQ	acetylation	[4, 5]
320	CSKGYSKKDFSSVFQ	succinylation	[5]
320	CSKGYSKKDFSSVFQ	ubiquitination	[2]

Reference

 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [11] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

  

Sequence Annotation

 NP_BIND 39 68 NAD (By similarity).
 NP_BIND 102 103 NAD (By similarity).
 ACT_SITE 208 208 By similarity.
 BINDING 107 107 NAD (By similarity).
 BINDING 133 133 NAD; via amide nitrogen (By similarity).
 BINDING 283 283 NAD (By similarity).  

Keyword

 Branched-chain amino acid catabolism; Complete proteome; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 335 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; ISS:UniProtKB.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0004616; F:phosphogluconate dehydrogenase (decarboxylating) activity; IEA:InterPro.
 GO:0006098; P:pentose-phosphate shunt; IEA:InterPro.
 GO:0006574; P:valine catabolic process; ISS:UniProtKB. 

Interpro

 IPR002204; 3-OH-isobutyrate_DH-rel_CS.
 IPR008927; 6-PGluconate_DH_C-like.
 IPR006115; 6PGDH_NADP-bd.
 IPR013328; DH_multihelical.
 IPR015815; HIBADH-type.
 IPR011548; IsoBut3OH_DH.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF03446; NAD_binding_2 

SMART

  

PROSITE

 PS00895; 3_HYDROXYISOBUT_DH 

PRINTS