CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028835
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 2-alpha kinase 4 
Protein Synonyms/Alias
  
Gene Name
 Eif2ak4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1258NSLCRLYKFIEQKGDacetylation[1]
1462DKEGSHVKVKSFEKEacetylation[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1648 AA 
Protein Sequence
MAGGRGASGR GRAEPQESYS QRQDHELQAL EAIYGSDFQD LRPDARGRVR EPPEINLVLY 60
PQGLAGEEVY VQVELRVKCP PTYPDVVPEI DLKNAKGLSN ESVNLLKSHL EELAKKQCGE 120
VMIFELAHHV QSFLSEHNKP PPKSFHEEML ERQAQEKQQR LLEARRKEEQ EQREILHEIQ 180
RRKEEIKEEK KRKEMAKQER LEITSLTNQD YASKRDPAGH RAAAILHGGS PDFVGNGKAR 240
TYSSGRSRRE RQYSVCSGEP SPGSCDILHF SVGSPDQLMV HKGRCVGSDE QLGKVVYNAL 300
ETATGSFVLL HEWVLQWQKM GPCLTSQEKE KIDKCKRQIQ GAETEFSSLV KLSHPNIVRY 360
FAMNSREEED SIVIDILAEH VSGISLATHL SHSGPVPAHQ LRKYTAQLLA GLDYLHSNSV 420
VHKVLSASSV LVDAEGTVKI TDYSISKRLA DICKEDVFEQ ARVRFSDSAL PYKTGKKGDV 480
WRLGLLLLSL SQGQECGEYP VTIPSDLPAD FQDFLKKCVC LDDKERWSPQ QLLKHSFINP 540
QPKLPLVEQS PEDSGGQDYI ETVIPSNQLP SAAFFSETQK QFSRYFIEFE ELQLLGKGAF 600
GAVIKVQNKL DGCCYAVKRI PINPASRHFR RIKGEVTLLS RLHHENIVRY YNAWIERHER 660
PAVPGTPPPD CTPQAQDSPA TCGKTSGDTE ELGSVEAAAP PPILSSSVEW STSAERSTST 720
RFPVTGQDSS SDEEDEDERD GVFSQSFLPA SDSDSDIIFD NEDENSKSQN QDEDCNQKDG 780
SHEIEPSVTA EAVHYLYIQM EYCEKSTLRD TIDQGLFRDT SRLWRLFREI LDGLAYIHEK 840
GMIHRDLKPV NIFLDSDDHV KIGDFGLATD HLAFTAEGKQ DDQAGDGVIK SDPSGHLTGM 900
VGTALYVSPE VQGSTKSAYN QKVDLFSLGI IFFEMSYHPM VTASERIFVL NQLRDPTSPK 960
FPDDFDDGEH TKQKSVISWL LNHDPAKRPT AMELLKSELL PPPQMEESEL HEVLHHTLAN 1020
IDGKAYRTMM SQIFCQHISP AIDYTYDSDI LKGNFLIRTA KIQQLVCETI VRVFKRHGAV 1080
QLCTPLLLPR NRQIYEHNEA ALFMDHSGML VMLPFDLRVP FARYVARNNI LNLKRYCIER 1140
VFRPRKLDRF HPKELLECAF DIVTSTTNSS LPTAETIYTI YEIIQEFPAL QERNYSIYLN 1200
HTMLLKAILL HCGIPEDKLS QVYVILYDAV TEKLTRREVE AKFCNLSLSS NSLCRLYKFI 1260
EQKGDLQDLT PTINSLIKQK TGVAQLVKYS LKDLEDVVGL LKKLGVKLQV SINLGLVYKV 1320
QQHTGIIFQF LAFSKRRQRV VPEILAAGGR YDLLIPKFRG PQTVGPVPTA VGVSIAIDKI 1380
FAAVLNMEEP VTVSSCDLLV VSVGQMSMSR AINLTQKLWT AGITAEIMYD WSQSQEELQE 1440
YCRHHEITYV ALVSDKEGSH VKVKSFEKER QTEKRVLESD LVDHVMQKLR TKVGDERNFR 1500
DASDNLAVQT LKGSFSNASG LFEIHGTTVV PNVIVLAPEK LSASTRRRHE IQVQTRLQTT 1560
LANLHQKSSE IEILAVDLPK ETILQFLSLE WDADEQAFNT TVKQLLSRLP KQRYLKLVCD 1620
EIYNIKVEKK VSVLFLYSYR DDYYRILF 1648 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004694; F:eukaryotic translation initiation factor 2alpha kinase activity; IEA:InterPro.
 GO:0009267; P:cellular response to starvation; IDA:MGI.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:MGI.
 GO:0017148; P:negative regulation of translation; IGI:MGI.
 GO:0043558; P:regulation of translational initiation in response to stress; IDA:MGI. 
Interpro
 IPR004154; Anticodon-bd.
 IPR026920; EIF2AK4.
 IPR024435; HisRS-related_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR006575; RWD-domain.
 IPR008271; Ser/Thr_kinase_AS.
 IPR016255; Ser/Thr_kinase_GCN2.
 IPR016135; UBQ-conjugating_enzyme/RWD. 
Pfam
 PF12745; HGTP_anticodon2
 PF00069; Pkinase
 PF05773; RWD 
SMART
 SM00591; RWD 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50908; RWD 
PRINTS