CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029469
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 NF-kappa-B essential modulator 
Protein Synonyms/Alias
  
Gene Name
 IKBKG 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111VERLGLEKLDLKRQKubiquitination[1]
164SRLEAATKECQALEGubiquitination[1]
275AKQEVIDKLKEEAEQubiquitination[2]
277QEVIDKLKEEAEQHKubiquitination[1, 3, 4]
301KAQADIYKADFQAERubiquitination[1, 4, 5, 6]
336QREYSKLKASCQESAubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 411 AA 
Protein Sequence
MNRHLWKSQL CEMVQPSGGP AADQDVLGEE SPLGKPAMLH LPSEQGAPET LQRCLEENQE 60
LRDAIRQSNQ ILRERCEELL HFQASQREEK EFLMCKFQEA RKLVERLGLE KLDLKRQKEQ 120
ALREVEHLKR CQQMAEDKAS VKAQVTSLLG ELQESQSRLE AATKECQALE GRARAASEQA 180
RQLESEREAL QQQHSVQVDQ LRMQGQSVEA ALRMERQAAS EEKRKLAQLQ VAYHQLFQEY 240
DNHIKSSVGM QLEDLKQQLQ QAEEALVAKQ EVIDKLKEEA EQHKIVMETV PVLKAQADIY 300
KADFQAERQA REKLAEKKEL LQEQLEQLQR EYSKLKASCQ ESARIEDMRK RHVEVSQAPL 360
PPAPAYLSSP LALPSQRRSP PEEPPDFCCP KCQYQAPDMD TLQIHVMECI E 411 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA. 
Interpro
 IPR021063; NEMO_N. 
Pfam
 PF11577; NEMO 
SMART
  
PROSITE
  
PRINTS