CPLM-006635

Genbank Nucleotide ID

ADP-ribosylation factor-like protein 3

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
11	LSILRKLKSAPDQEV	ubiquitination	[1, 2]
35	AGKTTLLKQLASEDI	ubiquitination	[3]
54	PTQGFNIKSVQSQGF	ubiquitination	[3]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]

Functional Description

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.

NP_BIND 24 31 GTP (By similarity).
NP_BIND 67 71 GTP (By similarity).
NP_BIND 126 129 GTP (By similarity).
NP_BIND 159 161 GTP (By similarity).
METAL 31 31 Magnesium (By similarity).
METAL 48 48 Magnesium (By similarity).
BINDING 48 48 GTP (By similarity).
BINDING 70 70 GTP; via amide nitrogen (By similarity).
MOD_RES 5 5 Phosphoserine.
LIPID 2 2 N-myristoyl glycine (Potential).

Cell cycle; Cell division; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Golgi apparatus; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005813; C:centrosome; IDA:UniProtKB.
GO:0005881; C:cytoplasmic microtubule; IDA:UniProtKB.
GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0030496; C:midbody; IDA:UniProtKB.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0032391; C:photoreceptor connecting cilium; IDA:UniProtKB.
GO:0005886; C:plasma membrane; IDA:HPA.
GO:0005876; C:spindle microtubule; IDA:UniProtKB.
GO:0019003; F:GDP binding; IDA:UniProtKB.
GO:0005525; F:GTP binding; IDA:UniProtKB.
GO:0003924; F:GTPase activity; IEA:Compara.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0008017; F:microtubule binding; IDA:UniProtKB.
GO:0060271; P:cilium morphogenesis; IMP:UniProtKB.
GO:0000910; P:cytokinesis; IMP:UniProtKB.
GO:0042073; P:intraflagellar transport; IEA:Compara.
GO:0001822; P:kidney development; ISS:UniProtKB.
GO:0042461; P:photoreceptor cell development; ISS:UniProtKB.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0007264; P:small GTPase mediated signal transduction; IDA:UniProtKB.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR024156; Small_GTPase_ARF.
IPR006689; Small_GTPase_ARF/SAR.