UniProt Accession

 THIF_ECOLI; P30138; P76780; Q2M8S9 

Genbank Protein ID

 M88701; U00006; U00096; AP009048 

Genbank Nucleotide ID

 AAB95618.1; AAC43090.1; AAC76966.2; BAE77327.1 

Protein Name

 Sulfur carrier protein ThiS adenylyltransferase 

Protein Synonyms/Alias

  

Gene Name

 thiF 

Gene Synonyms/Alias

 b3992; JW3956 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
113	RLTGEALKDAVARAD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS. 

Sequence Annotation

 NP_BIND 127 131 ATP.
 ACT_SITE 184 184 Glycyl persulfide ester intermediate.
 METAL 169 169 Zinc.
 METAL 172 172 Zinc.
 METAL 240 240 Zinc.
 METAL 243 243 Zinc.
 BINDING 11 11 ATP.
 BINDING 38 38 ATP; via amide nitrogen.
 BINDING 59 59 ATP.
 BINDING 70 70 ATP.
 BINDING 83 83 ATP.
 BINDING 107 107 ATP; via amide nitrogen.
 CROSSLNK 184 184 Glycyl cysteine dithioester (Cys-Gly)  

Keyword

 3D-structure; ATP-binding; Complete proteome; Metal-binding; Nucleotide-binding; Nucleotidyltransferase; Reference proteome; Thiamine biosynthesis; Thioester bond; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 251 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
 GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
 GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR012731; Adenyl_ThiF.
 IPR007901; MoeZ_MoeB.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 

Pfam

 PF05237; MoeZ_MoeB
 PF00899; ThiF 

SMART

  

PROSITE

  

PRINTS