CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005807
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GMP synthase [glutamine-hydrolyzing] 
Protein Synonyms/Alias
 GMP synthetase; Glutamine amidotransferase 
Gene Name
 guaA 
Gene Synonyms/Alias
 BSU06360 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
46TLTAEEIKKMNPKGIacetylation[1]
505VVYDITSKPPATIEWacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 Catalyzes the synthesis of GMP from XMP (By similarity). 
Sequence Annotation
 DOMAIN 8 198 Glutamine amidotransferase type-1.
 DOMAIN 199 388 GMPS ATP-PPase.
 NP_BIND 227 233 ATP (By similarity).
 ACT_SITE 85 85 Nucleophile (By similarity).
 ACT_SITE 172 172 By similarity.
 ACT_SITE 174 174 By similarity.  
Keyword
 ATP-binding; Complete proteome; Glutamine amidotransferase; GMP biosynthesis; Ligase; Nucleotide-binding; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MTKLVNEMIL VLDFGSQYNQ LITRRIREFG VYSELHPHTL TAEEIKKMNP KGIILSGGPN 60
SVYDENSFRC DEKIFELDIP VLGICYGMQL MTHYLGGKVE AASQREYGKA NIRIEGTPDL 120
FRDLPNEQVV WMSHGDLVVE VPEGFTVDAT SHHCPNSAMS KADKKWYGVQ FHPEVRHSEY 180
GNDLLKNFVF GVCECEGEWS MENFIEIEMQ KIRETVGDKQ VLCALSGGVD SSVVAVLIHK 240
AIGDQLTCIF VDHGLLRKGE AEGVMKTFSE GFNMNVIKVD AKDRFLNKLK GVSDPEQKRK 300
IIGNEFIYVF DDEADKLKGI DYLAQGTLYT DIIESGTATA QTIKSHHNVG GLPEDMQFEL 360
IEPLNTLFKD EVRALGTELG IPDEIVWRQP FPGPGLGIRV LGEVTEEKLE IVRESDAILR 420
EEIANHGLER DIWQYFTVLP DIRSVGVMGD ARTYDYTIGI RAVTSIDGMT SDWARIPWDV 480
LEVISTRIVN EVKHINRVVY DITSKPPATI EWE 513 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003922; F:GMP synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
 GO:0016462; F:pyrophosphatase activity; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IEA:HAMAP.
 GO:0006177; P:GMP biosynthetic process; IEA:HAMAP. 
Interpro
 IPR017926; GATASE.
 IPR001674; GMP_synth_C.
 IPR004739; GMP_synth_N.
 IPR022955; GMP_synthase.
 IPR025777; GMPS_ATP_PPase_dom.
 IPR022310; NAD/GMP_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00117; GATase
 PF00958; GMP_synt_C
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1
 PS51553; GMPS_ATP_PPASE 
PRINTS