UniProt Accession

 KCC4_RAT; P13234; A1A5L5; Q63892 

Genbank Protein ID

 M63334; M74488; M64757; M64757; S65840; BC128706; J04600; J04446 

Genbank Nucleotide ID

 AAA40865.1; AAA40845.1; AAA40856.1; AAA40857.1; AAB28372.1; AAI28707.1; AAA41867.1; AAA40990.1 

Protein Name

 Calcium/calmodulin-dependent protein kinase type IV 

Protein Synonyms/Alias

 CaMK IV; CaM kinase-GR; Calspermin 

Gene Name

 Camk4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
66	CKQKGTQKPYALKVL	acetylation	[1]
186	IADFGLSKIVEHQVL	acetylation	[1]
311	VHMDTAQKKLQEFNA	acetylation	[1]
312	HMDTAQKKLQEFNAR	acetylation	[1]
327	RKLKAAVKAVVASSR	acetylation	[1]
362	AQPAQDGKDKTDPLE	acetylation	[1]
381	AGDHEAAKAAADETM	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Isoform 1: Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18 (By similarity). 

Sequence Annotation

 DOMAIN 42 296 Protein kinase.
 NP_BIND 48 56 ATP (By similarity).
 REGION 297 336 Autoinhibitory domain.
 REGION 302 319 PP2A-binding (By similarity).
 REGION 318 337 Calmodulin-binding (Potential).
 ACT_SITE 160 160 Proton acceptor (By similarity).
 BINDING 71 71 ATP (By similarity).
 MOD_RES 11 11 Phosphoserine; by autocatalysis (By
 MOD_RES 12 12 Phosphoserine; by autocatalysis (By
 MOD_RES 196 196 Phosphothreonine; by CaMKK1 and CaMKK2
 MOD_RES 332 332 Phosphoserine; by autocatalysis.
 CARBOHYD 53 53 O-linked (GlcNAc) (By similarity).
 CARBOHYD 54 54 O-linked (GlcNAc) (By similarity).
 CARBOHYD 133 133 O-linked (GlcNAc) (By similarity).
 CARBOHYD 185 185 O-linked (GlcNAc) (By similarity).
 CARBOHYD 340 340 O-linked (GlcNAc) (By similarity).
 CARBOHYD 341 341 O-linked (GlcNAc) (By similarity).
 CARBOHYD 352 352 O-linked (GlcNAc) (By similarity).  

Keyword

 Adaptive immunity; Alternative promoter usage; Alternative splicing; ATP-binding; Calcium; Calmodulin-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycoprotein; Immunity; Inflammatory response; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 474 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IDA:RGD.
 GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
 GO:0007616; P:long-term memory; ISS:UniProtKB.
 GO:0043011; P:myeloid dendritic cell differentiation; ISS:UniProtKB.
 GO:0007270; P:neuron-neuron synaptic transmission; IEA:Compara.
 GO:0006913; P:nucleocytoplasmic transport; IEA:Compara.
 GO:0046827; P:positive regulation of protein export from nucleus; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0007165; P:signal transduction; IEA:Compara. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS