UniProt Accession

 TS101_HUMAN; Q99816; Q9BUM5 

Genbank Protein ID

 U82130; BC002487 

Genbank Nucleotide ID

 AAC52083.1; AAH02487.1 

Protein Name

 Tumor susceptibility gene 101 protein 

Protein Synonyms/Alias

 ESCRT-I complex subunit TSG101 

Gene Name

 TSG101 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
90	NPPICFVKPTSSMTI	acetylation	[1]
237	LISAVSDKLRWRMKE	ubiquitination	[2, 3, 4, 5]
257	QAELNALKRTEEDLK	ubiquitination	[2, 4, 5]
361	IDLDVFLKHVRLLSR	ubiquitination	[2, 4, 5]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. 

Sequence Annotation

 DOMAIN 2 145 UEV.
 DOMAIN 322 390 SB.
 REGION 158 162 Interaction with CEP55.
 MOTIF 320 323 PTAP motif.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Endosome; Growth regulation; Host-virus interaction; Membrane; Nucleus; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 390 AA 

Protein Sequence

Gene Ontology

 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0000813; C:ESCRT I complex; IDA:UniProtKB.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005771; C:multivesicular body; TAS:HGNC.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0043130; F:ubiquitin binding; TAS:HGNC.
 GO:0007050; P:cell cycle arrest; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006464; P:cellular protein modification process; IEA:InterPro.
 GO:0046788; P:egress of virus within host cell; TAS:Reactome.
 GO:0016197; P:endosomal transport; TAS:Reactome.
 GO:0030216; P:keratinocyte differentiation; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0046755; P:non-lytic virus budding; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0001558; P:regulation of cell growth; IEA:Compara.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IMP:UniProtKB.
 GO:0019082; P:viral protein processing; TAS:Reactome.
 GO:0019068; P:virion assembly; TAS:Reactome. 

Interpro

 IPR017916; Steadiness_box.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR008883; UEV_N. 

Pfam

 PF05743; UEV
 PF09454; Vps23_core 

SMART

  

PROSITE

 PS51312; SB
 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2
 PS51322; UEV 

PRINTS