CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031981
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytosolic purine 5'-nucleotidase 
Protein Synonyms/Alias
 cDNA FLJ53335, highly similar to Cytosolic purine 5'-nucleotidase (EC 3.1.3.5) 
Gene Name
 NT5C2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111TREQYPNKFIQRDDTubiquitination[1, 2, 3]
157TSCETGFKDGDLFMSubiquitination[2]
182AVDWVHYKGSLKEKTubiquitination[1, 2, 4, 5]
186VHYKGSLKEKTVENLubiquitination[2]
188YKGSLKEKTVENLEKubiquitination[2]
199NLEKYVVKDGKLPLLubiquitination[2]
202KYVVKDGKLPLLLSRubiquitination[2, 4, 5, 6]
215SRMKEVGKVFLATNSubiquitination[1]
225LATNSDYKYTDKIMTubiquitination[1]
263LILVDARKPLFFGEGubiquitination[2, 3]
313ICDLLGAKGKDILYIubiquitination[3, 6]
315DLLGAKGKDILYIGDubiquitination[2, 4, 5]
330HIFGDILKSKKRQGWubiquitination[2, 4]
486NRTSVDFKDTDYKRHubiquitination[2, 4]
491DFKDTDYKRHQLTRSubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
 ACT_SITE 23 23 Nucleophile (By similarity).
 ACT_SITE 25 25 Proton donor (By similarity).
 METAL 23 23 Magnesium (By similarity).
 METAL 25 25 Magnesium; via carbonyl oxygen (By
 METAL 322 322 Magnesium (By similarity).  
Keyword
 Complete proteome; Magnesium; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 532 AA 
Protein Sequence
MSKEGVFVNR SLAMEKIKCF GFDMDYTLAV YKSPEYESLG FELTVERLVS IGYPQELLSF 60
AYDSTFPTRG LVFDTLYGNL LKVDAYGNLL VCAHGFNFIR GPETREQYPN KFIQRDDTER 120
FYILNTLFNL PETYLLACLV DFFTNCPRYT SCETGFKDGD LFMSYRSMFQ DVRDAVDWVH 180
YKGSLKEKTV ENLEKYVVKD GKLPLLLSRM KEVGKVFLAT NSDYKYTDKI MTYLFDFPHG 240
PKPGSSHRPW QSYFDLILVD ARKPLFFGEG TVLRQVDTKT GKLKIGTYTG PLQHGIVYSG 300
GSSDTICDLL GAKGKDILYI GDHIFGDILK SKKRQGWRTF LVIPELAQEL HVWTDKSSLF 360
EELQSLDIFL AELYKHLDSS SNERPDISSI QRRIKKVTHD MDMCYGMMGS LFRSGSRQTL 420
FASQVMRYAD LYAASFINLL YYPFSYLFRA AHVLMPHEST VEHTHVDINE MESPLATRNR 480
TSVDFKDTDY KRHQLTRSIS EIKPPNLFPL APQEITHCHD EDDDEEEEEE EE 532 
Gene Ontology
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 
Interpro
 IPR023214; HAD-like_dom.
 IPR008380; HAD-SF_hydro_IG_5-nucl.
 IPR016695; Pur_nucleotidase. 
Pfam
 PF05761; 5_nucleotid 
SMART
  
PROSITE
  
PRINTS