UniProt Accession

 AK1A1_RAT; P51635 

Genbank Protein ID

 D10854; BC059133 

Genbank Nucleotide ID

 BAA01627.1; AAH59133.1 

Protein Name

 Alcohol dehydrogenase [NADP(+)] 

Protein Synonyms/Alias

 3-DG-reducing enzyme; Aldehyde reductase; Aldo-keto reductase family 1 member A1 

Gene Name

 Akr1a1 

Gene Synonyms/Alias

 Alr 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
13	VLLHTGQKMPLIGLG	acetylation	[1]
23	LIGLGTWKSEPGQVK	acetylation	[1]
23	LIGLGTWKSEPGQVK	glycation	[2]
34	GQVKAAIKYALSVGY	acetylation	[1]
68	KESVGAGKAVPREEL	acetylation	[1]
68	KESVGAGKAVPREEL	glycation	[2]
85	TSKLWNTKHHPEDVE	acetylation	[1]
85	TSKLWNTKHHPEDVE	glycation	[2]
127	RGDNPFPKNADGTVK	acetylation	[1]
134	KNADGTVKYDSTHYK	acetylation	[1]
141	KYDSTHYKETWKALE	acetylation	[1]
141	KYDSTHYKETWKALE	glycation	[2]
145	THYKETWKALEALVA	acetylation	[1]
153	ALEALVAKGLVKALG	acetylation	[1]
153	ALEALVAKGLVKALG	glycation	[2]
157	LVAKGLVKALGLSNF	acetylation	[1]
240	VVLALAEKHGRSPAQ	acetylation	[1]
263	RKVICIPKSITPSRI	acetylation	[1]
294	KQLDALNKNWRYIVP	acetylation	[1]
308	PMITVDGKRVPRDAG	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] In vivo glycation of aldehyde reductase, a major 3-deoxyglucosone reducing enzyme: identification of glycation sites.
 Takahashi M, Lu YB, Myint T, Fujii J, Wada Y, Taniguchi N.
 Biochemistry. 1995 Jan 31;34(4):1433-8. [PMID: 7827091] 

Functional Description

 Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs (By similarity). Catalyzes the NADPH-dependent reduction of 3-deoxyglucosone (3-DG). 

Sequence Annotation

 NP_BIND 11 20 NADP (Potential).
 NP_BIND 211 273 NADP (By similarity).
 ACT_SITE 50 50 Proton donor (By similarity).
 BINDING 113 113 Substrate (By similarity).
 MOD_RES 2 2 N-acetylthreonine.
 CARBOHYD 23 23 N-linked (Glc) (glycation).
 CARBOHYD 68 68 N-linked (Glc) (glycation).
 CARBOHYD 85 85 N-linked (Glc) (glycation).
 CARBOHYD 141 141 N-linked (Glc) (glycation).
 CARBOHYD 153 153 N-linked (Glc) (glycation).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Glycation; Glycoprotein; NADP; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0008106; F:alcohol dehydrogenase (NADP+) activity; IEA:EC.
 GO:0047939; F:L-glucuronate reductase activity; IEA:Compara.
 GO:0046185; P:aldehyde catabolic process; IEA:Compara.
 GO:0042840; P:D-glucuronate catabolic process; IEA:Compara.
 GO:0019853; P:L-ascorbic acid biosynthetic process; IEA:Compara. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.