UniProt Accession

 MPIP2_HUMAN; P30305; D3DVY1; D3DVY2; D3DVY3; D3DVY4; O43551; Q13971; Q5JX77; Q6RSS1; Q9BRA6 

Genbank Protein ID

 M81934; S78187; X96436; Z68092; AY494082; AL109804; AL109804; AL109804; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; CH471133; BC006395; BC009953; BC051711; AF036233; AF036233 

Genbank Nucleotide ID

 AAA58416.1; AAB21139.1; CAA65303.1; CAA92108.1; AAR26469.1; CAC17548.1; CAC17549.1; CAI18847.1; EAX10491.1; EAX10492.1; EAX10493.1; EAX10494.1; EAX10496.1; EAX10497.1; EAX10498.1; EAX10499.1; AAH06395.1; AAH09953.1; AAH51711.1; AAB94622.1; AAB94624.1 

Protein Name

 M-phase inducer phosphatase 2 

Protein Synonyms/Alias

 Dual specificity phosphatase Cdc25B 

Gene Name

 CDC25B 

Gene Synonyms/Alias

 CDC25HU2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
71	GLGSETPKSQVGTLL	ubiquitination	[1]
177	QAPDGRRKSEAGSGA	ubiquitination	[1]
204	FVFKMPWKPTHPSST	ubiquitination	[1]
242	MCLSPDRKMEVEELS	ubiquitination	[1, 2]
299	LISAPLVKTLEKEEE	ubiquitination	[3]
303	PLVKTLEKEEEKDLV	ubiquitination	[1, 2]
307	TLEKEEEKDLVMYSK	ubiquitination	[2]
334	SVIRPILKRLERPQD	ubiquitination	[1]
349	RDTPVQNKRRRSVTP	ubiquitination	[1]
374	KARVLRSKSLCHDEI	ubiquitination	[1]
398	ELIGDYSKAFLLQTV	ubiquitination	[2, 3, 4]
408	LLQTVDGKHQDLKYI	ubiquitination	[1, 2]
434	KFSNIVDKFVIVDCR	ubiquitination	[1, 2]
452	EYEGGHIKTAVNLPL	ubiquitination	[1, 2]
469	DAESFLLKSPIAPCS	ubiquitination	[1]
479	IAPCSLDKRVILIFH	ubiquitination	[1]
523	YPEMYILKGGYKEFF	ubiquitination	[2]
527	YILKGGYKEFFPQHP	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. 

Sequence Annotation

 DOMAIN 431 538 Rhodanese.
 ACT_SITE 487 487
 MOD_RES 169 169 Phosphoserine; by MELK.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 323 323 Phosphoserine; by MELK and MAPK14.
 MOD_RES 353 353 Phosphoserine; by AURKA.
 MOD_RES 375 375 Phosphoserine; by BRSK1 and MAPK14.  

Keyword

 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Mitosis; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 580 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; TAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007144; P:female meiosis I; IEA:Compara.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:UniProtKB.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0001556; P:oocyte maturation; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0032467; P:positive regulation of cytokinesis; IMP:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
 GO:0045860; P:positive regulation of protein kinase activity; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IDA:UniProtKB. 

Interpro

 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 

Pfam

 PF06617; M-inducer_phosp
 PF00581; Rhodanese 

SMART

 SM00450; RHOD 

PROSITE

 PS50206; RHODANESE_3 

PRINTS

 PR00716; MPIPHPHTASE.