CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005218
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 M-phase inducer phosphatase 
Protein Synonyms/Alias
 Mitosis initiation protein MIH1; Mitotic inducer homolog 
Gene Name
 MIH1 
Gene Synonyms/Alias
 YMR036C; YM9532.01C; YM9973.10C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
26SFQKISLKSPFGKKKacetylation[1]
406ESKRFATKNNSFRKLubiquitination[2]
412TKNNSFRKLASPSNPubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1. 
Sequence Annotation
 DOMAIN 261 373 Rhodanese.
 ACT_SITE 320 320 By similarity.  
Keyword
 Cell cycle; Cell division; Complete proteome; Hydrolase; Mitosis; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 554 AA 
Protein Sequence
MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS NVDDDLINKE 60
NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE NDDIVLSMHF ASQTLQSPTR 120
NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI 180
PRSSRKSSQK FSNITQNTLN FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF 240
FPRISPETLK NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 300
VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF YPDILILDGG 360
YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES KRFATKNNSF RKLASPSNPN 420
FFYRDSHQSS TTMASSALSF RFEPPPKLSL NHRRVSSGSS LNSSESTGDE NFFPILSKSS 480
MSSNSNLSTS HMLLMDGLDT PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA 540
RRSLFPSLET EDKK 554 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004725; F:protein tyrosine phosphatase activity; ISS:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IGI:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IMP:SGD. 
Interpro
 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 
Pfam
 PF00581; Rhodanese 
SMART
 SM00450; RHOD 
PROSITE
 PS50206; RHODANESE_3 
PRINTS
 PR00716; MPIPHPHTASE.