UniProt Accession

 SYT1_MOUSE; P46096 

Genbank Protein ID

 D37792; AK078790; BC042519 

Genbank Nucleotide ID

 BAA07040.1; BAC37395.1; AAH42519.1 

Protein Name

 Synaptotagmin-1 

Protein Synonyms/Alias

 Synaptotagmin I; SytI; p65 

Gene Name

 Syt1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
104	GKNAINMKDVKDLGK	ubiquitination	[1]
119	TMKDQALKDDDAETG	ubiquitination	[1]
133	GLTDGEEKEEPKEEE	ubiquitination	[1]
189	KVFLLPDKKKKFETK	ubiquitination	[1]
200	FETKVHRKTLNPVFN	ubiquitination	[1]
213	FNEQFTFKVPYSELG	ubiquitination	[1]
236	YDFDRFSKHDIIGEF	ubiquitination	[1]
267	RDLQSAEKEEQEKLG	ubiquitination	[1]
272	AEKEEQEKLGDICFS	ubiquitination	[1]
297	TVVILEAKNLKKMDV	ubiquitination	[1]
301	LEAKNLKKMDVGGLS	ubiquitination	[1]
313	GLSDPYVKIHLMQNG	ubiquitination	[1]
321	IHLMQNGKRLKKKKT	ubiquitination	[1]
332	KKKTTIKKNTLNPYY	ubiquitination	[1]
369	LDYDKIGKNDAIGKV	ubiquitination	[1]
375	GKNDAIGKVFVGYNS	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. 

Sequence Annotation

 DOMAIN 143 244 C2 1.
 DOMAIN 274 377 C2 2.
 REGION 135 381 Phospholipid binding (Probable).
 METAL 171 171 Calcium 2; via carbonyl oxygen (By
 METAL 172 172 Calcium 1 (By similarity).
 METAL 172 172 Calcium 2 (By similarity).
 METAL 178 178 Calcium 1 (By similarity).
 METAL 230 230 Calcium 1 (By similarity).
 METAL 230 230 Calcium 2 (By similarity).
 METAL 231 231 Calcium 1; via carbonyl oxygen (By
 METAL 232 232 Calcium 1 (By similarity).
 METAL 232 232 Calcium 2 (By similarity).
 METAL 232 232 Calcium 3 (By similarity).
 METAL 235 235 Calcium 3 (By similarity).
 METAL 236 236 Calcium 3; via carbonyl oxygen (By
 METAL 238 238 Calcium 2 (By similarity).
 METAL 238 238 Calcium 3 (By similarity).
 MOD_RES 128 128 Phosphothreonine.
 MOD_RES 229 229 Phosphotyrosine.
 MOD_RES 364 364 Phosphotyrosine.
 MOD_RES 380 380 Phosphotyrosine.
 LIPID 74 74 S-palmitoyl cysteine (By similarity).
 LIPID 75 75 S-palmitoyl cysteine (By similarity).
 LIPID 77 77 S-palmitoyl cysteine (By similarity).
 LIPID 79 79 S-palmitoyl cysteine (By similarity).
 LIPID 82 82 S-palmitoyl cysteine (By similarity).
 CARBOHYD 24 24 N-linked (GlcNAc...) (By similarity).  

Keyword

 Calcium; Cell junction; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; Glycoprotein; Lipoprotein; Membrane; Metal-binding; Palmitate; Phosphoprotein; Reference proteome; Repeat; Synapse; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 421 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042584; C:chromaffin granule membrane; IEA:UniProtKB-SubCell.
 GO:0031045; C:dense core granule; IEA:Compara.
 GO:0060076; C:excitatory synapse; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043005; C:neuron projection; IDA:MGI.
 GO:0005886; C:plasma membrane; TAS:UniProtKB.
 GO:0042734; C:presynaptic membrane; IDA:MGI.
 GO:0030141; C:secretory granule; IDA:MGI.
 GO:0030672; C:synaptic vesicle membrane; IDA:MGI.
 GO:0005509; F:calcium ion binding; IDA:MGI.
 GO:0005544; F:calcium-dependent phospholipid binding; TAS:MGI.
 GO:0050750; F:low-density lipoprotein particle receptor binding; ISO:MGI.
 GO:0005215; F:transporter activity; IEA:InterPro.
 GO:0017158; P:regulation of calcium ion-dependent exocytosis; IEA:Compara.
 GO:0016079; P:synaptic vesicle exocytosis; IMP:MGI. 

Interpro

 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR020477; C2_dom.
 IPR018029; C2_membr_targeting.
 IPR001565; Synaptotagmin.
 IPR015428; Synaptotagmin1. 

Pfam

 PF00168; C2 

SMART

 SM00239; C2 

PROSITE

 PS50004; C2 

PRINTS

 PR00360; C2DOMAIN.
 PR00399; SYNAPTOTAGMN.