| Tag | Content |
|---|
| CPLM ID | CPLM-023436 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 12 |
Protein Synonyms/Alias | Mediator complex subunit 12; Mediator complex subunit Kohtalo; dTRAP230 |
Gene Name | kto |
Gene Synonyms/Alias | Med12; Trap230; CG8491 |
Created Date | July 27, 2013 |
Organism | Drosophila melanogaster (Fruit fly) |
NCBI Taxa ID | 7227 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 2439 | QQSAGGGKPGVLNTQ | acetylation | [1] |
|
Reference | [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation. Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C. Sci Signal. 2011 Jul 26;4(183):ra48. [ PMID: 21791702] |
Functional Description | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for leg and eye development and macrochaete specification or differentiation. |
Sequence Annotation | MOD_RES 745 745 Phosphothreonine.
MOD_RES 748 748 Phosphoserine.
MOD_RES 781 781 Phosphoserine.
MOD_RES 806 806 Phosphoserine.
MOD_RES 1356 1356 Phosphoserine.
MOD_RES 1360 1360 Phosphothreonine. |
Keyword | Activator; Complete proteome; Developmental protein; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 2531 AA |
Protein Sequence | MLSMLQEKRP LKRTRLGPPD IYPQDAKQRE DELTPTNVKH GFTTTPPLSD EFGTAHNSNV 60
NASKVSAFFS GVLAKKEELM TLPDTGRKKQ QINCKDNFWP VSPRRKCTVD AWFKDLAGNK 120
PLLSLAKRAP SFNKKEEIFI TLCENQVNMQ RATWFIKLSA AYTLSFTESK NKKRSIYDPA 180
AEWTGNMIKF MKELLPKLQE YYQQNHDKSS SNGTTSGSLT AAGNGPASNG STGTSSINSV 240
TGSSASTNVI PVPSMASPLP PIHSPANGQQ AAPGGGVNAG SVMPTTGSLG GVVGGPGSSV 300
VGGAAGAGAA VPPGSTISGI GSQFEDSRNA LKYWKYCHQL SKYMYEESLL DRQEFLNWIL 360
DLLDKMRTQA SFDEPLKKLV LSFALQYMHD FVQSERLCRK MAYIVSKKLA QLLNTVVEQQ 420
TIKELDEPKL QQDPYELALQ EQMSCPHHRD IVLYLSTILQ IITIECPTAL VWSGIAAHRA 480
PSSLLGSPLD HLPLAPSVLP MPTRCPRTNH EIRRQLRAAE SDIVLRTQHA EQRWFAAKWL 540
SAGKNQYTSV LATLDHLDTH CFDRMEHNNS IDTLYAQIFP SPTVSRRREE DQVEPRPPYE 600
PKQDKDTVRI LCEWAVSGQR WGEHRAMVVA ILLDKRQIDV TSTPADQQSS DKDDKDSLAS 660
GAGLIDGLPV FQHVLMHFLD HDAPVLDEHV SSPQQRTEFT NLVQLFSALI RHDVFSHNAY 720
MHTLISRGDL LLESVLVIKS GTTATKTSPP PPAPPPTTTH GFDDDGFGGG LDFKHNEFDD 780
SNVDDDLDKL VQNIKEKGQQ HEAPDSPKIG PPGDGETNPG GSISRHYVYT KHFPIPQDDP 840
SMSSYSSESN QRYILLFGVG KERDEKKHAV KKMSKEIGKL FTKKFSIDVA AAGHVKKHSR 900
NEFNFEATTS KCQQMAYFDQ HVVTAQCAAN VLEQLNGFAL GNNNYLPVQE HVAFLFDLME 960
LALNIYSLLE LCDSLLKELP EVEHQLQLKK SNLVRSYTTS LALYIVSILR RYHSCLLLSP 1020
EQTLSVFEGV CRTIRHVSNP SECTSAERCI IAYLSDLHES CVLLQGKEQS TEYYQQLQCI 1080
KRFKDIFNTP EQLDLPPQGY NPLLLQELFM APRRGGKLDP HWLGTLHESP ANVYSFVSNA 1140
LIAVCRETDN ERLNDVALAC AELTASCNVL SEEWIYALQS LCSGSKSPRY PHLGGQVDIG 1200
QLKTHNALAV FVCILVARHC FSLADFVSKF ALPTLARSVS AGGAELSVDA EAGARLTCHL 1260
VLKLFKTLEI PQPGMYSVST SPNPLHAVGN DFSIRLSCDR HLLVGAHKTI PIAAVLAVLK 1320
AILIVVDNAA LKTPLASGSG TSSGGLGGAF GSGKRSGFNT PVHPGSTPKS NEQRPADLSQ 1380
ILGTSDLQLG SSLTSEPEAL QQPSVGGMEQ ISLLEFAQAV LKQICAQEHV LERCLKNAEQ 1440
LCDMIIDEML TAKQAQRVLH MICYPEPEFN IISELDQRSM IVRILENLGQ WTLRISWLDL 1500
QLMYRQSLSN NAELNVWLDT VARAAIDVFH MEEVVLPGAV KATHKPKPST WLVAPLIAKL 1560
TPAVQGRILR VAGQVLESMN YFSKVSKSDC NSSGSGDERE KSNSCHSSNS YGLGGVPARN 1620
KKMPLDYQPF LGLILTCLKG QDEYKENLLV SLYAQLSQCL QSFAELDTIG GIDEPQAREE 1680
ILDALQLRFS LVGGMFEAIQ KNSTPTTDWA ILLAQLVCQG VVDLSCNREL FTTVVDMLAT 1740
LVHSTLVSDD ERHYMNLMKK LKKEIGEKNN ASIRVIRQLL PLYKQPTEVI ACEHSGMDTK 1800
GNKICDIDKK QLRISDKQRI SVWDILEGHK NPAPLSWVWF GAVKLERKPL TYEEAHRNLK 1860
YHTHSLVKPS SYYYEPLPLP PEDIEPVPEK ICIKDEMKAD TPSSVDQSPS AVVGGTGRGR 1920
GKGTTTRKRK PKNPKTPPVV NTQQQQPQLA QQPQQPQNVQ QQQLQQQQQQ QQHMQQQHMQ 1980
QQQMQPNQMG QMPMNMPMNM QQFAPNPNNM MQQNAMLQQQ QQQQMQQMGN NPMQQQLNVG 2040
GGNGQPNPQM NFMQQGPGGG GAGPQGMPGQ QQQWHNAPQQ QQPPQPYHNQ YAPHQQNMQS 2100
NRIERPPLNA NSKQALSQML RQRQPFQQQA QQGPGGGFNP MQQQPQASQQ QPGPQQQMNP 2160
NQMRQQQMNP QQNPQSVAAF NAMQQQPQQN AQQQQMNPNQ QQQQQFMRGG NMRPGMAPNQ 2220
MNQMNMGGQG MSQNPMMQQQ IPQNMVGMVN PNANQMMQSG GAQGGNGVGV GVGVGVGGAG 2280
NNPNMGMGGM PQQGMIQQQP QQQPQQQVQF QNFQNQYQQQ QQQGMQQQGG GAGVGVGVGM 2340
APNQQQQQQA NMMGNFNPQM QQGNRNNPDF MAAAAVAQQQ QQQQQQQRVV PGGMMAGNRN 2400
QYMNQAPNVT MSTMMGPGPG GVVGQVPPYA RQQSAGGGKP GVLNTQQQFQ QQQQQQQQLR 2460
HQMMQLQGMG GGAGGGMGAG PQQGGGAVGG GAGGGMVPQQ QSMNQQQTPN LVAQLQRQNM 2520
MGQQQYQPPP Y 2531 |
Gene Ontology | GO:0016592; C:mediator complex; IDA:UniProtKB. GO:0001104; F:RNA polymerase II transcription cofactor activity; IDA:UniProtKB. GO:0022416; P:chaeta development; IMP:FlyBase. GO:0048749; P:compound eye development; IMP:FlyBase. GO:0036011; P:imaginal disc-derived leg segmentation; IMP:FlyBase. GO:0030177; P:positive regulation of Wnt receptor signaling pathway; IMP:FlyBase. GO:0045498; P:sex comb development; IMP:FlyBase. GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:FlyBase. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |