| Tag | Content |
|---|
| CPLM ID | CPLM-002256 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Eukaryotic peptide chain release factor GTP-binding subunit |
Protein Synonyms/Alias | ERF-3; ERF3; ERF2; G1 to S phase transition protein 1; Omnipotent suppressor protein 2; PSI no more protein 2; Polypeptide release factor 3; Translation release factor 3 |
Gene Name | SUP35 |
Gene Synonyms/Alias | GST1; PNM2; SAL3; SUF12; SUP2; YDR172W; YD9395.05 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 400 | AKTQGVNKMVVVVNK | ubiquitination | [1] | | 417 | DPTVNWSKERYDQCV | ubiquitination | [1] | | 679 | GTTIAIGKIVKIAE* | acetylation | [2] | | 679 | GTTIAIGKIVKIAE* | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides. Recruited by polyadenylate-binding protein PAB1 to poly(A)-tails of mRNAs. Interaction with PAB1 is also required for regulation of normal mRNA decay through translation termination-coupled poly(A) shortening. |
Sequence Annotation | NP_BIND 267 274 GTP (By similarity).
NP_BIND 344 348 GTP (By similarity).
NP_BIND 406 409 GTP (By similarity).
REGION 1 239 Interaction with PAB1.
REGION 5 135 Prion domain (PrD).
REGION 139 249 Charged.
MOD_RES 571 571 Phosphoserine. |
Keyword | 3D-structure; Amyloid; Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Phosphoprotein; Prion; Protein biosynthesis; Reference proteome; Repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 685 AA |
Protein Sequence | MSDSNQGNNQ QNYQQYSQNG NQQQGNNRYQ GYQAYNAQAQ PAGGYYQNYQ GYSGYQQGGY 60
QQYNPDAGYQ QQYNPQGGYQ QYNPQGGYQQ QFNPQGGRGN YKNFNYNNNL QGYQAGFQPQ 120
SQGMSLNDFQ KQQKQAAPKP KKTLKLVSSS GIKLANATKK VGTKPAESDK KEEEKSAETK 180
EPTKEPTKVE EPVKKEEKPV QTEEKTEEKS ELPKVEDLKI SESTHNTNNA NVTSADALIK 240
EQEEEVDDEV VNDMFGGKDH VSLIFMGHVD AGKSTMGGNL LYLTGSVDKR TIEKYEREAK 300
DAGRQGWYLS WVMDTNKEER NDGKTIEVGK AYFETEKRRY TILDAPGHKM YVSEMIGGAS 360
QADVGVLVIS ARKGEYETGF ERGGQTREHA LLAKTQGVNK MVVVVNKMDD PTVNWSKERY 420
DQCVSNVSNF LRAIGYNIKT DVVFMPVSGY SGANLKDHVD PKECPWYTGP TLLEYLDTMN 480
HVDRHINAPF MLPIAAKMKD LGTIVEGKIE SGHIKKGQST LLMPNKTAVE IQNIYNETEN 540
EVDMAMCGEQ VKLRIKGVEE EDISPGFVLT SPKNPIKSVT KFVAQIAIVE LKSIIAAGFS 600
CVMHVHTAIE EVHIVKLLHK LEKGTNRKSK KPPAFAKKGM KVIAVLETEA PVCVETYQDY 660
PQLGRFTLRD QGTTIAIGKI VKIAE 685 |
Gene Ontology | GO:0005829; C:cytosol; TAS:Reactome. GO:0018444; C:translation release factor complex; IDA:SGD. GO:0005525; F:GTP binding; IEA:UniProtKB-KW. GO:0003924; F:GTPase activity; IEA:InterPro. GO:0003747; F:translation release factor activity; IDA:SGD. GO:0006184; P:GTP catabolic process; IEA:GOC. GO:0000288; P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; IPI:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |