CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012249
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosome biogenesis protein BOP1 
Protein Synonyms/Alias
 Block of proliferation 1 protein 
Gene Name
 BOP1 
Gene Synonyms/Alias
 KIAA0124 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
161LDGRRIYKPLRTRDEubiquitination[1]
175ELDQFLDKMDDPDYWubiquitination[2]
253FIPSLVEKEKVSRMVubiquitination[1, 2, 3, 4]
255PSLVEKEKVSRMVHAubiquitination[1]
264SRMVHAIKMGWIQPRubiquitination[1]
339QQEPGERKLSFLPRKubiquitination[1]
391DPEDLIPKLPRPRDLubiquitination[1, 2, 3, 4, 5]
615LRQELTKKLMPNCKWubiquitination[1]
621KKLMPNCKWVSSLAVubiquitination[1, 5]
654FDLDLSTKPYRMLRHubiquitination[2, 6]
711LVPVKVLKGHVLTRDubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. 
Sequence Annotation
 REPEAT 411 450 WD 1.
 REPEAT 452 492 WD 2.
 REPEAT 532 576 WD 3.
 REPEAT 577 615 WD 4.
 REPEAT 618 657 WD 5.
 REPEAT 661 700 WD 6.
 REPEAT 716 746 WD 7.
 REGION 265 427 Sufficient for nucleolar localization (By
 MOD_RES 106 106 Phosphothreonine.
 MOD_RES 126 126 Phosphoserine (By similarity).
 MOD_RES 127 127 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribosome biogenesis; rRNA processing; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 746 AA 
Protein Sequence
MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF 60
SGLEDSGSDS SEDDDEGDEE GEDGALDDEG HSGIKKTTEE QVQASTPCPR TEMASARIGD 120
EYAEDSSDEE DIRNTVGNVP LEWYDDFPHV GYDLDGRRIY KPLRTRDELD QFLDKMDDPD 180
YWRTVQDPMT GRDLRLTDEQ VALVRRLQSG QFGDVGFNPY EPAVDFFSGD VMIHPVTNRP 240
ADKRSFIPSL VEKEKVSRMV HAIKMGWIQP RRPRDPTPSF YDLWAQEDPN AVLGRHKMHV 300
PAPKLALPGH AESYNPPPEY LLSEEERLAW EQQEPGERKL SFLPRKFPSL RAVPAYGRFI 360
QERFERCLDL YLCPRQRKMR VNVDPEDLIP KLPRPRDLQP FPTCQALVYR GHSDLVRCLS 420
VSPGGQWLVS GSDDGSLRLW EVATARCVRT VPVGGVVKSV AWNPSPAVCL VAAAVEDSVL 480
LLNPALGDRL VAGSTDQLLS AFVPPEEPPL QPARWLEASE EERQVGLRLR ICHGKPVTQV 540
TWHGRGDYLA VVLATQGHTQ VLIHQLSRRR SQSPFRRSHG QVQRVAFHPA RPFLLVASQR 600
SVRLYHLLRQ ELTKKLMPNC KWVSSLAVHP AGDNVICGSY DSKLVWFDLD LSTKPYRMLR 660
HHKKALRAVA FHPRYPLFAS GSDDGSVIVC HGMVYNDLLQ NPLLVPVKVL KGHVLTRDLG 720
VLDVIFHPTQ PWVFSSGADG TVRLFT 746 
Gene Ontology
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0070545; C:PeBoW complex; IDA:UniProtKB.
 GO:0008283; P:cell proliferation; NAS:UniProtKB.
 GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:UniProtKB.
 GO:0051726; P:regulation of cell cycle; IMP:UniProtKB. 
Interpro
 IPR012953; BOP1_N_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08145; BOP1NT
 PF00400; WD40 
SMART
 SM01035; BOP1NT
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS