CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024032
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear receptor coactivator 3 
Protein Synonyms/Alias
 NCoA-3; ACTR; Amplified in breast cancer 1 protein; AIB-1; CBP-interacting protein; pCIP; Class E basic helix-loop-helix protein 42; bHLHe42; Receptor-associated coactivator 3; RAC-3; Steroid receptor coactivator protein 3; SRC-3; Thyroid hormone receptor activator molecule 1; TRAM-1 
Gene Name
 NCOA3 
Gene Synonyms/Alias
 AIB1; BHLHE42; RAC3; TRAM1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
316DGQSWSQKRHYQEAYubiquitination[1]
616QRGPLESKGHKKLLQacetylation[2]
619PLESKGHKKLLQLLTacetylation[2]
620LESKGHKKLLQLLTCacetylation[2]
687EKHRILHKLLQNGNSacetylation[3]
723CGDGNVVKQEQLSPKsumoylation[4]
786QEKDPKIKTETSEEGsumoylation[4]
1194SRQALELKMENPTAGsumoylation[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase.
 Chen H, Lin RJ, Xie W, Wilpitz D, Evans RM.
 Cell. 1999 Sep 3;98(5):675-86. [PMID: 10490106]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Coordinated regulation of AIB1 transcriptional activity by sumoylation and phosphorylation.
 Wu H, Sun L, Zhang Y, Chen Y, Shi B, Li R, Wang Y, Liang J, Fan D, Wu G, Wang D, Li S, Shang Y.
 J Biol Chem. 2006 Aug 4;281(31):21848-56. [PMID: 16760465] 
Functional Description
 Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9. 
Sequence Annotation
 DOMAIN 25 82 bHLH.
 DOMAIN 110 180 PAS.
 REGION 1023 1093 Interaction with CREBBP.
 REGION 1097 1304 Acetyltransferase.
 MOTIF 685 689 LXXLL motif 1.
 MOTIF 738 742 LXXLL motif 2.
 MOTIF 1057 1061 LXXLL motif 3.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 551 551 Phosphoserine.
 MOD_RES 601 601 Phosphoserine; by CK1.
 MOD_RES 616 616 N6-acetyllysine; by CREBBP.
 MOD_RES 619 619 N6-acetyllysine; by CREBBP.
 MOD_RES 620 620 N6-acetyllysine; by CREBBP.
 MOD_RES 687 687 N6-acetyllysine.
 MOD_RES 728 728 Phosphoserine.
 MOD_RES 857 857 Phosphoserine.
 MOD_RES 867 867 Phosphoserine.
 MOD_RES 1042 1042 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Activator; Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Methylation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1424 AA 
Protein Sequence
MSGLGENLDP LASDSRKRKL PCDTPGQGLT CSGEKRRREQ ESKYIEELAE LISANLSDID 60
NFNVKPDKCA ILKETVRQIR QIKEQGKTIS NDDDVQKADV SSTGQGVIDK DSLGPLLLQA 120
LDGFLFVVNR DGNIVFVSEN VTQYLQYKQE DLVNTSVYNI LHEEDRKDFL KNLPKSTVNG 180
VSWTNETQRQ KSHTFNCRML MKTPHDILED INASPEMRQR YETMQCFALS QPRAMMEEGE 240
DLQSCMICVA RRITTGERTF PSNPESFITR HDLSGKVVNI DTNSLRSSMR PGFEDIIRRC 300
IQRFFSLNDG QSWSQKRHYQ EAYLNGHAET PVYRFSLADG TIVTAQTKSK LFRNPVTNDR 360
HGFVSTHFLQ REQNGYRPNP NPVGQGIRPP MAGCNSSVGG MSMSPNQGLQ MPSSRAYGLA 420
DPSTTGQMSG ARYGGSSNIA SLTPGPGMQS PSSYQNNNYG LNMSSPPHGS PGLAPNQQNI 480
MISPRNRGSP KIASHQFSPV AGVHSPMASS GNTGNHSFSS SSLSALQAIS EGVGTSLLST 540
LSSPGPKLDN SPNMNITQPS KVSNQDSKSP LGFYCDQNPV ESSMCQSNSR DHLSDKESKE 600
SSVEGAENQR GPLESKGHKK LLQLLTCSSD DRGHSSLTNS PLDSSCKESS VSVTSPSGVS 660
SSTSGGVSST SNMHGSLLQE KHRILHKLLQ NGNSPAEVAK ITAEATGKDT SSITSCGDGN 720
VVKQEQLSPK KKENNALLRY LLDRDDPSDA LSKELQPQVE GVDNKMSQCT SSTIPSSSQE 780
KDPKIKTETS EEGSGDLDNL DAILGDLTSS DFYNNSISSN GSHLGTKQQV FQGTNSLGLK 840
SSQSVQSIRP PYNRAVSLDS PVSVGSSPPV KNISAFPMLP KQPMLGGNPR MMDSQENYGS 900
SMGGPNRNVT VTQTPSSGDW GLPNSKAGRM EPMNSNSMGR PGGDYNTSLP RPALGGSIPT 960
LPLRSNSIPG ARPVLQQQQQ MLQMRPGEIP MGMGANPYGQ AAASNQLGSW PDGMLSMEQV 1020
SHGTQNRPLL RNSLDDLVGP PSNLEGQSDE RALLDQLHTL LSNTDATGLE EIDRALGIPE 1080
LVNQGQALEP KQDAFQGQEA AVMMDQKAGL YGQTYPAQGP PMQGGFHLQG QSPSFNSMMN 1140
QMNQQGNFPL QGMHPRANIM RPRTNTPKQL RMQLQQRLQG QQFLNQSRQA LELKMENPTA 1200
GGAAVMRPMM QPQVSSQQGF LNAQMVAQRS RELLSHHFRQ QRVAMMMQQQ QQQQQQQQQQ 1260
QQQQQQQQQQ QQQQQQTQAF SPPPNVTASP SMDGLLAGPT MPQAPPQQFP YQPNYGMGQQ 1320
PDPAFGRVSS PPNAMMSSRM GPSQNPMMQH PQAASIYQSS EMKGWPSGNL ARNSSFSQQQ 1380
FAHQGNPAVY SMVHMNGSSG HMGQMNMNPM PMSGMPMGPD QKYC 1424 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0004402; F:histone acetyltransferase activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0046966; F:thyroid hormone receptor binding; NAS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0048589; P:developmental growth; IEA:Compara.
 GO:0060744; P:mammary gland branching involved in thelarche; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0035624; P:receptor transactivation; TAS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0060068; P:vagina development; IEA:Compara. 
Interpro
 IPR011598; bHLH_dom.
 IPR010011; DUF1518.
 IPR009110; Nuc_rcpt_coact.
 IPR014920; Nuc_rcpt_coact_Ncoa-typ.
 IPR017426; Nuclear_rcpt_coactivator.
 IPR000014; PAS.
 IPR013767; PAS_fold.
 IPR014935; SRC-1.
 IPR008955; Src1_rcpt_coact. 
Pfam
 PF07469; DUF1518
 PF08815; Nuc_rec_co-act
 PF00989; PAS
 PF08832; SRC-1 
SMART
 SM00353; HLH
 SM00091; PAS 
PROSITE
 PS50888; BHLH
 PS50112; PAS 
PRINTS