CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043375
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 RNA-binding protein FUS 
Protein Synonyms/Alias
  
Gene Name
 FUS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
264SDRGGFNKFGGPRDQubiquitination[1]
317GIIKTNKKTGQPMINubiquitination[2, 3, 4]
333YTDRETGKLKGEATVacetylation[1, 5]
335DRETGKLKGEATVSFubiquitination[1, 4]
349FDDPPSAKAAIDWFDubiquitination[3, 6]
358AIDWFDGKEFSGNPIubiquitination[1, 4]
366EFSGNPIKVSFATRRubiquitination[2, 4, 6]
449RNECNQCKAPKPDGPubiquitination[1, 4]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 527 AA 
Protein Sequence
MASNDYTQQA TQSYGAYPTQ PGQGYSQQSS QPYGQQSYSG YSQSTDTSGY GQSSYSSYGQ 60
SQNTGYGTQS TPQGYGSTGG YGSSQSSQSS YGQQSSYPGY GQQPAPSSTS GSYGSSSQSS 120
SYGQPQSGSY SQQPSYGGQQ QSYGQQQSYN PPQGYGQQNQ YNSSSGGGGG GGGGGNYGQD 180
QSSMSSGGGS GGGYGNQDQS GGGGSGGYGQ QDRGGRGRGG SGGGGGGGGG GYNRSSGGYE 240
PRGRGGGRGG RGGMGGSDRG GFNKFGGPRD QGSRHDSAEQ DNSDNNTIFV QGLGENVTIE 300
SVADYFKQIG IIKTNKKTGQ PMINLYTDRE TGKLKGEATV SFDDPPSAKA AIDWFDGKEF 360
SGNPIKVSFA TRRADFNRGG GNGRGGRGRG GPMGRGGYGG GGSGGGGRGG FPSGGGGGGG 420
QQRAGDWKCP NPTCENMNFS WRNECNQCKA PKPDGPGGGP GGSHMGGNYG DDRRGGRGGY 480
DRGGYRGRGG DRGGFRGGRG GGDRGGFGPG KMDSRGEHRQ DRRERPY 527 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR001876; Znf_RanBP2. 
Pfam
 PF00076; RRM_1
 PF00641; zf-RanBP 
SMART
 SM00360; RRM
 SM00547; ZnF_RBZ 
PROSITE
 PS50102; RRM
 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2 
PRINTS