| Tag | Content |
|---|
| CPLM ID | CPLM-002470 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Saccharopepsin |
Protein Synonyms/Alias | Aspartate protease; PrA; Proteinase A; Carboxypeptidase Y-deficient protein 4; Proteinase YSCA |
Gene Name | PEP4 |
Gene Synonyms/Alias | PHO9; PRA1; YPL154C; P2585 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 60 | KYLTQFEKANPEVVF | ubiquitination | [1] | | 318 | NAEIGAKKGWTGQYT | ubiquitination | [1] | | 387 | VGDAFLRKYYSIYDL | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Aspartyl protease implicated in the post-translational regulation of S.cerevisiae vacuolar proteinases. Acts on YSCB, on YSCY and on itself. |
Sequence Annotation | ACT_SITE 109 109 By similarity.
ACT_SITE 294 294 By similarity.
CARBOHYD 144 144 N-linked (GlcNAc...).
CARBOHYD 345 345 N-linked (GlcNAc...).
DISULFID 122 127
DISULFID 328 361 |
Keyword | 3D-structure; Aspartyl protease; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome; Signal; Vacuole; Zymogen. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 405 AA |
Protein Sequence | MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL GQKYLTQFEK 60
ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP PQNFKVILDT GSSNLWVPSN 120
ECGSLACFLH SKYDHEASSS YKANGTEFAI QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE 180
ATSEPGLTFA FGKFDGILGL GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE 240
NGGEATFGGI DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI 300
TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP YDYTLEVSGS 360
CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG LAKAI 405 |
Gene Ontology | GO:0000328; C:fungal-type vacuole lumen; TAS:SGD. GO:0005739; C:mitochondrion; IDA:SGD. GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW. GO:0008233; F:peptidase activity; IDA:SGD. GO:0009267; P:cellular response to starvation; IMP:SGD. GO:0016237; P:microautophagy; IMP:SGD. GO:0051603; P:proteolysis involved in cellular protein catabolic process; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |