CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015706
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine--tRNA ligase 
Protein Synonyms/Alias
 Tyrosyl-tRNA synthetase; TyrRS 
Gene Name
 tyrS 
Gene Synonyms/Alias
 STM1449 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
235ITKADGTKFGKTEGGacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 
Sequence Annotation
 DOMAIN 357 414 S4 RNA-binding.
 MOTIF 42 51 "HIGH" region.
 MOTIF 235 239 "KMSKS" region.
 BINDING 37 37 Tyrosine (By similarity).
 BINDING 175 175 Tyrosine (By similarity).
 BINDING 179 179 Tyrosine (By similarity).
 BINDING 238 238 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 424 AA 
Protein Sequence
MASSNLIKQL QERGLVAQVT DEDALAERLA QGPIALYCGF DPTADSLHLG HLVPLLCLKR 60
FQQAGHKPVA LVGGATGLIG DPSFKAAERK LNTEETVQEW VAKIRKQVAP FLDFDCGENS 120
AIAANNYDWF GSMNVLTFLR DIGKHFSVNQ MINKEAVKQR LNRDDQGISF TEFSYNLLQG 180
YDFACLNKLH GVALQIGGSD QWGNITSGID LTRRLHQNQV FGLTVPLITK ADGTKFGKTE 240
GGAVWLDPKK TSPYKFYQFW INTADADVYR FLKFFTFMDI EEINALEEED KNSGKAPRAQ 300
YVLAEQVTRL VHGEEGLVAA KRITECLFSG SLSALSEADF EQLAQDGVPM VEMEKGADLM 360
QALVDAELQP SRGQARKTIA SNAVTINGEK QSDPEYIFND EDRLFGRYTL LRRGKKNYCL 420
ICWK 424 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:HAMAP.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type.
 IPR024107; Tyr-tRNA-ligase_bac_1. 
Pfam
 PF01479; S4
 PF00579; tRNA-synt_1b 
SMART
 SM00363; S4 
PROSITE
 PS00178; AA_TRNA_LIGASE_I
 PS50889; S4 
PRINTS
 PR01040; TRNASYNTHTYR.