CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA fragmentation factor subunit alpha 
Protein Synonyms/Alias
 DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; ICAD 
Gene Name
 DFFA 
Gene Synonyms/Alias
 DFF1; DFF45; H13 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
189REEVRQSKQLLQLYLubiquitination[1, 2]
208KEGSLLSKQEESKAAubiquitination[3, 4]
248ILTALREKQAPELSLubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Inhibitor of the caspase-activated DNase (DFF40). 
Sequence Annotation
 DOMAIN 17 96 CIDE-N.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 315 315 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 331 AA 
Protein Sequence
MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT 60
LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA 120
GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD 180
QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH 240
ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE RELALRLQQT 300
QSLHSLRSIS ASKASPPGDL QNPKRARQDP T 331 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004536; F:deoxyribonuclease activity; TAS:ProtInc.
 GO:0006309; P:apoptotic DNA fragmentation; IDA:MGI.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0035556; P:intracellular signal transduction; TAS:ProtInc.
 GO:0043066; P:negative regulation of apoptotic process; IDA:BHF-UCL. 
Interpro
 IPR003508; CAD.
 IPR027296; DFF-C_dom.
 IPR017299; DNA_fragmentation_factor_asu.
 IPR015121; DNA_fragmentation_mid_dom. 
Pfam
 PF02017; CIDE-N
 PF09033; DFF-C 
SMART
 SM00266; CAD 
PROSITE
 PS51135; CIDE_N 
PRINTS