UniProt Accession

 NO66_HUMAN; Q9H6W3; B4DT02 

Genbank Protein ID

 AY390535; AK025455; AK299994; AC005280; BC011350; BC071954 

Genbank Nucleotide ID

 AAR27292.1; BAB15138.1; BAG61814.1; AAH11350.1; AAH71954.1 

Protein Name

 Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 

Protein Synonyms/Alias

 60S ribosomal protein L8 histidine hydroxylase; Histone lysine demethylase NO66; Myc-associated protein with JmjC domain; Nucleolar protein 66; hsNO66; Ribosomal oxygenase NO66; ROX 

Gene Name

 NO66 

Gene Synonyms/Alias

 C14orf169; MAPJD 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
480	RRTAFMEKVRVLVAR	ubiquitination	[1]
503	AVADQRAKDFIHDSL	ubiquitination	[1, 2]
635	DKGLLLTKMPLALN*	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC- induced transcriptional activation. 

Sequence Annotation

 DOMAIN 294 439 JmjC.
 METAL 340 340 Iron; catalytic (By similarity).
 METAL 342 342 Iron; catalytic (By similarity).
 METAL 405 405 Iron; catalytic (By similarity).
 MOD_RES 109 109 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Chromatin regulator; Complete proteome; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 641 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0051864; F:histone demethylase activity (H3-K36 specific); ISS:UniProtKB.
 GO:0032453; F:histone demethylase activity (H3-K4 specific); ISS:UniProtKB.
 GO:0005506; F:iron ion binding; ISS:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0045668; P:negative regulation of osteoblast differentiation; ISS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR003347; JmjC_dom.
 IPR013109; NO66/MINA. 

Pfam

 PF08007; Cupin_4 

SMART

 SM00558; JmjC 

PROSITE

 PS51184; JMJC 

PRINTS